Document Detail


Taking tissue-engineering principles into theater: augmentation of impacted allograft with human bone marrow stromal cells.
MedLine Citation:
PMID:  17465735     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human bone marrow contains bone progenitor cells that arise from multipotent mesenchymal stem cells. Seeding bone progenitor cells onto a scaffold can produce a 3D living composite with significant mechanical and biological potential. This article details laboratory and clinical findings from two clinical cases, where different proximal femoral conditions were treated using impacted allograft augmented with marrow-derived autogenous progenitor cells. Autologous bone marrow was seeded onto highly washed morselized allograft and impacted. Samples of the impacted graft were also taken for ex vivo analysis. Both patients made an uncomplicated clinical recovery. Imaging confirmed defect filling with encouraging initial graft incorporation. Histochemical and alkaline phosphatase staining demonstrated that a live composite graft with osteogenic activity had been introduced into the defects. These studies demonstrate that marrow-derived cells can adhere to highly washed morselized allograft, survive the impaction process and proliferate with an osteoblastic phenotype, thus creating a living composite.
Authors:
Simon Tilley; Benjamin J R F Bolland; Kris Partridge; Andrew M R New; Jeremy M Latham; Douglas G Dunlop; Richard O C Oreffo
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regenerative medicine     Volume:  1     ISSN:  1746-076X     ISO Abbreviation:  -     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278116     Medline TA:  Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  685-92     Citation Subset:  IM    
Affiliation:
Bone & Joint Research Group, Developmental Origins of Health and Disease, University of Southampton, Southampton, UK. roco@soton.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Alkaline Phosphatase / metabolism
Bone Density
Bone Marrow Cells / cytology*
Bone Marrow Transplantation
Bone Remodeling
Bone Transplantation
Cell Adhesion
Cell Proliferation
Colony-Forming Units Assay
Ethidium / metabolism
Femur Neck / injuries*,  pathology,  radiography,  surgery
Follow-Up Studies
Fractures, Bone
Graft Survival
Hip / radiography
Histocytochemistry
Humans
Immunohistochemistry
Male
Middle Aged
Osteoblasts / physiology
Pelvis / radiography
Stress, Mechanical
Stromal Cells / cytology,  transplantation*
Time Factors
Tissue Engineering / methods*
Transplantation, Homologous
Treatment Outcome
Chemical
Reg. No./Substance:
3546-21-2/Ethidium; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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