Document Detail


Tailor-made cell patterning using a near-infrared-responsive composite gel composed of agarose and carbon nanotubes.
MedLine Citation:
PMID:  23302183     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Micropatterning is useful for regulating culture environments. We developed a highly efficient near-infrared-(NIR)-responsive gel and established a new technique that enables cell patterning by NIR irradiation. As a new culture substratum, we designed a tissue culture plate that was coated with a composite gel composed of agarose and carbon nanotubes (CNTs). A culture plate coated with agarose only showed no response to NIR irradiation. In contrast, NIR laser irradiation induced heat generation by CNTs; this permitted local solation of the CNT/agarose gel, and consequently, selective cell-adhesive regions were exposed on the tissue culture plate. The solation area was controlled by the NIR intensity, magnification of the object lens and CNT concentration in the gel. Furthermore, we formed circular patterns of HeLa cells and linear patterns of 3T3 cells on the same culture plate through selective and stepwise NIR irradiation of the CNT/agarose gel, and we also demonstrated that individual 3T3 cells migrated along a linear path formed on the CNT/agarose gel by NIR irradiation. These results indicate that our technique is useful for tailor-made cell patterning of stepwise and/or complex cell patterns, which has various biological applications such as stepwise co-culture and the study of cell migration.
Authors:
Haruka Koga; Takao Sada; Tsuyohiko Fujigaya; Naotoshi Nakashima; Kohji Nakazawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Biofabrication     Volume:  5     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  -    
Other Details:
Languages:  ENG     Pagination:  015010     Citation Subset:  -    
Affiliation:
Department of Life and Environment Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan. Research Fellow of the Japan Society for the Promotion of Science (JSPS), 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan.
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