Document Detail


Tactile sensory input regulates basal and apomorphine-induced immediate-early gene expression in rat barrel cortex.
MedLine Citation:
PMID:  8077463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clipping of mystacial vibrissae on one side of the rat's snout results in sensorimotor asymmetries in normal behavior and in behavior induced by the dopamine receptor agonist, apomorphine. Immediate-early gene expression, a marker for short-term changes in neuron function, was used to examine whether this sensory deprivation leads to functional changes in the somatosensory barrel cortex under experimental conditions which reveal behavioral asymmetries. The expression of c-fos and zif268 immediate-early genes was assessed with in situ hybridization histochemistry. Four hours after unilateral clipping of the mystacial vibrissae, the level of zif268 mRNA was reduced in the corresponding part of the contralateral barrel field. Injection of apomorphine (5 mg/kg) resulted in increased expression of both c-fos and zif268 immediate-early genes in cortex and striatum. This apomorphine-induced increase was blocked in the sensory-deprived somatosensory cortex. Laminar analysis of gene regulation showed that vibrissae removal affected immediate-early gene expression in all layers of the barrel cortex. These results demonstrate that: (1) basal zif268 gene expression in neurons of the somatosensory cortex is dependent on sensory input, (2) cortical immediate-early gene expression is increased after dopamine receptor activation, and (3) in the barrel cortex, this increase is also dependent on sensory input. We suggest that the observed reduction in gene expression after vibrissae removal reflects decreased activation of neurons in the barrel column by removal of sensory input.
Authors:
H Steiner; C R Gerfen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  344     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-10-03     Completed Date:  1994-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Affiliation:
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apomorphine / pharmacology*
Autoradiography
Base Sequence
Behavior, Animal / drug effects
DNA-Binding Proteins / genetics
Early Growth Response Protein 1
Gene Expression / drug effects*,  physiology
Genes, Immediate-Early*
Immediate-Early Proteins*
Male
Molecular Sequence Data
Oligonucleotide Probes / genetics
Proto-Oncogene Proteins c-fos / genetics
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Somatosensory Cortex / drug effects,  physiology*
Touch / physiology*
Transcription Factors / genetics
Vibrissae / physiology*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Egr1 protein, rat; 0/Immediate-Early Proteins; 0/Oligonucleotide Probes; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 0/Transcription Factors; 58-00-4/Apomorphine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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