| TWIST interacts with β-catenin signaling on osteosarcoma cell survival against cisplatin. | |
| | |
MedLine Citation:
|
PMID: 23280703 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Both TWIST and Wnt/β-catenin signaling reportedly play important roles in osteosarcoma development. In the present study, we explored the regulatory effect of TWIST on β-catenin in osteosarcoma cells and assessed how the functional interaction between TWIST and β-catenin would impact osteosarcoma cell survival against chemotherapy agent cisplatin. Overexpression and knockdown of TWIST were respectively performed in Saos-2 and MG-63 osteosarcoma cells. Overexpression of TWIST in Saos-2 cells significantly decreased the soluble β-catenin level, phosphorylation of glycogen synthase kinase-3β (GSK-3β) at serine 9, the mRNA level of β-catenin signaling target genes, and cell survival against cisplatin, which was strengthened by knocking down β-catenin. Knockdown of TWIST in MG-63 cells significantly increased the soluble β-catenin level, phosphorylation of GSK-3β at serine 9, the mRNA level of β-catenin signaling target genes, and cell survival against cisplatin, which was reversed by knocking down β-catenin or phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002. In conclusion, we demonstrate that TWIST decreases osteosarcoma cell survival against cisplatin by decreasing the soluble β-catenin level through a PI3K-dependent manner. This study provides the first evidence of a functional link between TWIST and β-catenin signaling in osteosarcoma cells, which adds fresh insights into the molecular mechanism of osteosarcoma development. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Jianhuang Wu; Qiande Liao; Hongbo He; Da Zhong; Ke Yin |
Related Documents
:
|
22990203 - Clca2, a target of the p53 family, negatively regulates cancer cell migration and invas... 23501253 - Cultured c2c12 cell lines as a model for assessment of bacterial attachment to bovine p... 12495673 - Type iv pili are not specifically required for contact dependent translocation of exoen... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-31 |
Journal Detail:
|
Title: Molecular carcinogenesis Volume: - ISSN: 1098-2744 ISO Abbreviation: Mol. Carcinog. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2013-1-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8811105 Medline TA: Mol Carcinog Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Spine, Xiangya Hospital, Central South University, Changsha, Hunan, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: UNCERTAINTY AND THE DECISION MAKER: ASSESSING AND MANAGING THE RISK OF UNDESIRABLE OUTCOMES.
Next Document: Immunoglobulins M survive Low-pH conditions used for virus inactivation and for elution from bioaffi...