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TWIST interacts with β-catenin signaling on osteosarcoma cell survival against cisplatin.
MedLine Citation:
PMID:  23280703     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Both TWIST and Wnt/β-catenin signaling reportedly play important roles in osteosarcoma development. In the present study, we explored the regulatory effect of TWIST on β-catenin in osteosarcoma cells and assessed how the functional interaction between TWIST and β-catenin would impact osteosarcoma cell survival against chemotherapy agent cisplatin. Overexpression and knockdown of TWIST were respectively performed in Saos-2 and MG-63 osteosarcoma cells. Overexpression of TWIST in Saos-2 cells significantly decreased the soluble β-catenin level, phosphorylation of glycogen synthase kinase-3β (GSK-3β) at serine 9, the mRNA level of β-catenin signaling target genes, and cell survival against cisplatin, which was strengthened by knocking down β-catenin. Knockdown of TWIST in MG-63 cells significantly increased the soluble β-catenin level, phosphorylation of GSK-3β at serine 9, the mRNA level of β-catenin signaling target genes, and cell survival against cisplatin, which was reversed by knocking down β-catenin or phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002. In conclusion, we demonstrate that TWIST decreases osteosarcoma cell survival against cisplatin by decreasing the soluble β-catenin level through a PI3K-dependent manner. This study provides the first evidence of a functional link between TWIST and β-catenin signaling in osteosarcoma cells, which adds fresh insights into the molecular mechanism of osteosarcoma development. © 2012 Wiley Periodicals, Inc.
Authors:
Jianhuang Wu; Qiande Liao; Hongbo He; Da Zhong; Ke Yin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-31
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  -     ISSN:  1098-2744     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Spine, Xiangya Hospital, Central South University, Changsha, Hunan, China.
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