| The TTF-1/TAP26 complex differentially modulates surfactant protein-B (SP-B) and -C (SP-C) promoters in lung cells. | |
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MedLine Citation:
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PMID: 16630564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Surfactant protein-B (SP-B) and -C (SP-C) are small hydrophobic surfactant proteins that maintain surface tension in alveoli. Both SP-B and SP-C are regulated by a key factor, thyroid transcription factor-1 (TTF-1), in lung cells. Previously, we identified a 26-kDa, TTF-1-associated protein (TAP26) that was shown to interact with TTF-1 and enhance TTF-1-transactivated SP-B promoter activity. In this study, we hypothesized that TAP26 could also serve as a co-activator of the SP-C promoter. Using the chromatin immunoprecipitation assay (ChIP), we demonstrated that TAP26 was not only a component of the SP-B promoter, but was also a component of the SP-C promoter complex in lung cells. TAP26 could synergistically stimulate TTF-1-activated SP-B and SP-C promoter activities in H441 cells (a lung adenocarcinoma cell). However, in MLE12 cells (a murine lung type II cell), only SP-B, but not SP-C, promoter activity was improved by TAP26 in a concentration-dependent manner. This result indicated that the TTF-1/TAP26 complex-activated SP-C promoter activity was already optimized in MLE12 cells and that the response of the SP-C promoter to the complex was different from that of the SP-B promoter. Via promoter mutation analysis, adjacent TTF-1 binding sites within the proximal promoter region of SP-C were found to be essential for TTF-1/TAP26-enhanced SP-C promoter activity. Thus, a dimerized complex structure was needed for advanced promoter activity. This result also provided a molecular mechanism by which both the SP-B and SP-C promoters could be differentially regulated by the same complex. |
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Authors:
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Meng-Chun Yang; Yuhong Guo; Chia-Chi Liu; Jonathan C Weissler; Yih-Sheng Yang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-04-04 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 344 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-01 Completed Date: 2006-06-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 484-90 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Pulmonary and Critical Care Medicine, UT Southwestern Medical Center, Dallas, TX 75390-9034, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Humans Lung / metabolism* Mice Nuclear Proteins / genetics, metabolism* Promoter Regions, Genetic / genetics Pulmonary Surfactant-Associated Protein B / genetics, metabolism* Pulmonary Surfactant-Associated Protein C / genetics, metabolism* Transcription Factors / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01HL63525/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCDC59 protein, human; 0/Nuclear Proteins; 0/Pulmonary Surfactant-Associated Protein B; 0/Pulmonary Surfactant-Associated Protein C; 0/Transcription Factors; 0/thyroid nuclear factor 1 |
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