Document Detail

TTF-1 and p63 for distinguishing pulmonary small-cell carcinoma from poorly differentiated squamous cell carcinoma in previously pap-stained cytologic material.
MedLine Citation:
PMID:  16680154     Owner:  NLM     Status:  MEDLINE    
In histology and cell block sections, antibodies to thyroid transcription factor-1 (TTF-1) and p63 have been demonstrated to be useful markers for distinguishing between small-cell lung carcinoma and poorly differentiated pulmonary squamous cell carcinoma. In this study, we assessed the utility of TTF-1 and p63, as an antibody panel, for differentiating between these two neoplasms in previously Papanicolaou (Pap)-stained cytologic smears and cytospin slides. Twenty-six lung carcinomas (13 small-cell lung carcinomas, 13 poorly differentiated pulmonary squamous cell carcinomas) were evaluated. One or two previously 95% ethanol-fixed, Pap-stained smears or cytospin slides were selected from each case. The cytologic material from these slides was transferred to positively charged slides. Unstained recuts were obtained from the corresponding histologic specimens or cell blocks. Immunohistochemical staining for TTF-1 and p63 was performed on the paired samples from each tumor. All (13/13) small-cell lung carcinomas were negative for p63 and 92% (12/13) were positive for TTF-1. Conversely, all (13/13) poorly differentiated pulmonary squamous cell carcinomas expressed p63 and did not express TTF-1. Immunoreactivity for p63 was also noted in bronchial reserve cells and metaplastic squamous cells. The immunostaining results obtained from the cytology slides were concordant with those of the histology or cell block sections in all cases. The results of this study show that TTF-1 and p63 immunostaining can be successfully applied to previously Pap-stained cytologic material, as an antibody panel, to facilitate pathologic differentiation between small-cell lung carcinomas and poorly differentiated pulmonary squamous cell carcinomas. p63 immunostaining, however, must be interpreted in conjunction with cytomorphology to distinguish between poorly differentiated pulmonary squamous cell carcinomas and benign cellular constituents of the lung.
Neda Kalhor; Dani S Zander; Jing Liu
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Publication Detail:
Type:  Journal Article     Date:  2006-05-05
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  19     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-20     Completed Date:  2006-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1117-23     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston Medical School, Houston, 77030, USA.
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MeSH Terms
Carcinoma, Small Cell / metabolism*,  pathology
Carcinoma, Squamous Cell / metabolism*,  pathology
Diagnosis, Differential
Immunoenzyme Techniques
Lung Neoplasms / metabolism*,  pathology
Membrane Proteins / metabolism*
Middle Aged
Nuclear Proteins / metabolism*
Staining and Labeling
Transcription Factors / metabolism*
Tumor Markers, Biological / metabolism
Reg. No./Substance:
0/CKAP4 protein, human; 0/Membrane Proteins; 0/Nuclear Proteins; 0/Transcription Factors; 0/Tumor Markers, Biological; 0/thyroid nuclear factor 1

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