Document Detail


A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth.
MedLine Citation:
PMID:  21816899     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MicroRNA (miRNA or miR) are an important class of regulators that participate in such biological functions as development, cell proliferation, differentiation, and apoptosis. The aim of this study was to elucidate the role of miRNA in cell proliferation using a unique cell system, namely thyroid cells that require thyrotropin for their growth. Here, we report the identification of a set of five specific miRNA (miR-1, miR-28-A, miR-290-5p, miR-296-3p, and miR-297a), whose down-regulation by thyrotropin is required for thyroid cell growth. In fact, overexpression of these miRNA negatively affects cell growth. We show that three of these miRNA target cAMP-responsive element binding protein (CREB)1, a thyrotropin-activated transcription factor, and that CREB1 binds the regulatory regions of the down-regulated miRNA. Hence, these data indicate that a synergistic loop involving thyrotropin, CREB1, and miRNA is required for thyroid cell proliferation.
Authors:
Vincenza Leone; Daniela D'Angelo; Angelo Ferraro; Pierlorenzo Pallante; Ileana Rubio; Massimo Santoro; Carlo Maria Croce; Alfredo Fusco
Related Documents :
17638909 - Doxorubicin affects testicular lipids with long-chain (c18-c22) and very long-chain (c2...
6356869 - Sertoli cells of the golden-mantled ground squirrel (spermophilus lateralis): a model s...
12398799 - Nucleus replacement in mammalian oocytes.
7504459 - Induction of germ-cell alkaline phosphatase by butyrate and cyclic amp in bewo chorioca...
17722739 - Toxicity of pharmaceutical wastewater on male reproductive system of mus musculus.
8954719 - Purification of primordial germ cells from tnapbeta-geo mouse embryos using facs-gal.
21501369 - The thermolysin-like metalloproteinase and virulence factor lasb from pathogenic pseudo...
1151269 - Differentiation in vitro of sympathetic cells from chick embryo sensory ganglia.
7415789 - A rapid method for the isolation and culture of endometrial epithelial cells responsive...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-4
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  -     ISSN:  1944-9917     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Istituto di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina e Chirurgia di Napoli (V.L., D.D., A.Fe., P.P., M.S., A.Fu.), Università degli Studi di Napoli "Federico II," Naples 80131, Italy; Naples Oncogenomic Center (V.L., A.Fe., P.P., M.S., A.Fu.), CEINGE (Centro Ingegneria Genetica)-Biotecnologie Avanzate, Naples, and European School of Molecular Medicine, Naples 80145, Italy; Department of Biological Sciences (I.R.), Federal University of São Paulo, São Paulo 05403, Brazil; Department of Molecular Virology (C.M.C.), Immunology, and Medical Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  p38 and p42/44 MAPKs differentially regulate progesterone receptor A and B isoform stabilization.
Next Document:  Estrogen sulfotransferase inhibits adipocyte differentiation.