Document Detail


TRP channel gene expression in the mouse retina.
MedLine Citation:
PMID:  22037305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to identify candidate cation channels important for retinal physiology, 28 TRP channel genes were surveyed for expression in the mouse retina. Transcripts for all TRP channels were detected by RT-PCR and sequencing. Northern blotting revealed that mRNAs for 12 TRP channel genes are enriched in the retina. The strongest signals were observed for TRPC1, TRPC3, TRPM1, TRPM3, and TRPML1, and clear signals were obtained for TRPC4, TRPM7, TRPP2, TRPV2, and TRPV4. In situ hybridization and immunofluorescence revealed widespread expression throughout multiple retinal layers for TRPC1, TRPC3, TRPC4, TRPML1, PKD1, and TRPP2. Striking localization of enhanced mRNA expression was observed for TRPC1 in the photoreceptor inner segment layer, for TRPM1 in the inner nuclear layer (INL), for TRPM3 in the INL, and for TRPML1 in the outer plexiform and nuclear layers. Strong immunofluorescence signal in cone outer segments was observed for TRPM7 and TRPP2. TRPC5 immunostaining was largely confined to INL cells immediately adjacent to the inner plexiform layer. TRPV2 antibodies stained photoreceptor axons in the outer plexiform layer. Expression of TRPM1 splice variants was strong in the ciliary body, whereas TRPM3 was strongly expressed in the retinal pigmented epithelium.
Authors:
Jared C Gilliam; Theodore G Wensel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-20
Journal Detail:
Title:  Vision research     Volume:  51     ISSN:  1878-5646     ISO Abbreviation:  Vision Res.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-28     Completed Date:  2012-01-25     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  0417402     Medline TA:  Vision Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  2440-52     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Gene Expression Profiling / methods*
Mice
Mice, Inbred C57BL
Polymerase Chain Reaction / methods
RNA, Messenger / metabolism
Retina / metabolism*
Retinal Pigment Epithelium / metabolism
Transient Receptor Potential Channels / genetics*,  metabolism
Grant Support
ID/Acronym/Agency:
P30 EY002520/EY/NEI NIH HHS; P30-EY002520/EY/NEI NIH HHS; P41 RR002250/RR/NCRR NIH HHS; R01 EY007981/EY/NEI NIH HHS; R01 EY011900/EY/NEI NIH HHS; R01-EY07981/EY/NEI NIH HHS; R01-EY11900/EY/NEI NIH HHS; T32 EY007001/EY/NEI NIH HHS; T32-EY007001/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Transient Receptor Potential Channels
Comments/Corrections

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