Document Detail


TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis.
MedLine Citation:
PMID:  17933793     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The patterning of epidermal cell types in Arabidopsis is a simple and useful model for studying the molecular basis of cell specification in plants. The distribution of different cell types in the Arabidopsis epidermis is regulated by a lateral inhibition mechanism that relies on interactions between transcription factors. However, it is unclear how temporal- or organ-specific differences in epidermal patterning are achieved. Here we identify TRICHOMELESS1 (TCL1) as a new and major single-repeat MYB-type transcription factor that negatively regulates trichome formation in the inflorescence epidermis. A dominant mutant with elevated expression of TCL1 has a glabrous (trichomeless) phenotype, whereas a loss-of-function mutation in TCL1 uniquely confers ectopic trichome formation on inflorescence stem and pedicels. Genetic analyses demonstrate that TCL1 and CAPRICE work synergistically to regulate trichome patterning on these organs. Interestingly, overexpression of TCL1 specifically suppresses the expression of GLABRA1 (GL1), a crucial component in the trichome initiation complex, whereas loss-of-function of TCL1 enhances GL1 expression. Chromatin immunoprecipitation results show that TCL1 can be recruited to the cis-acting regulatory elements of GL1. These results provide the first molecular and genetic evidence that an R3 MYB may negatively regulate trichome cell specification in a novel manner by directly suppressing the transcription of GL1.
Authors:
Shucai Wang; Su-Hwan Kwak; Qingning Zeng; Brian E Ellis; Xiao-Ya Chen; John Schiefelbein; Jin-Gui Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  134     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-15     Completed Date:  2008-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3873-82     Citation Subset:  IM    
Affiliation:
Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Arabidopsis / genetics,  growth & development*,  metabolism*
Arabidopsis Proteins / chemistry,  genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Down-Regulation*
Gene Expression Regulation, Plant*
Molecular Sequence Data
Mutation / genetics
Phylogeny
Plants, Genetically Modified
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA-Binding Proteins; 0/Myb protein, Arabidopsis; 0/TCL1 protein, Arabidopsis; 0/Transcription Factors; 144417-31-2/GL1 protein, Arabidopsis

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