Document Detail

TPX2 in malignantly transformed human bronchial epithelial cells by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.
MedLine Citation:
PMID:  18723071     Owner:  NLM     Status:  MEDLINE    
In order to elucidate the function of the targeting protein for Xenopus kinesin-like protein 2 (Xklp2) (TPX2) in the malignant transformation of human bronchial epithelial cells induced by anti-benzo[a]pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide (anti-BPDE), TPX2 was characterized in cells at both the gene and the protein levels. TPX2 was present at higher levels in 16HBE-C cells than in 16HBE cells as demonstrated by two-dimensional gel electrophoresis, immunocytochemistry, Western blot analysis and RT-PCR. TPX2 was also detected in lung squamous-cell carcinoma tissues by immunohistochemistry, but not in normal lung tissues. Depression of TPX2 by RNA interference in 16HBE-C cells led to a decrease in cell proliferation, S-phase cell cycle arrest and cell apoptosis. Abnormal TPX2 tyrosine phosphorylation was detected in 16HBE-C cells, and this could be inhibited, to different degrees, by tyrosine kinase inhibitors. Inhibiting tyrosine phosphorylation in 16HBE-C cells by three selected tyrosine protein kinase inhibitors, tyrphostin 47, AG112 and AG555, caused G(0)/G(1)-phase cell cycle arrest. Our results suggest that anti-BPDE can cause the over-expression of TPX2 and its aberrant tyrosine phosphorylation. Misregulation of TPX2 affects the cell cycle state, proliferation rates and apoptosis.
Lijuan Zhang; He Huang; Luyao Deng; Ming Chu; Lan Xu; Juanling Fu; Yunlan Zhu; Xiuchun Zhang; Shulin Liu; Zongcan Zhou; Yuedan Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-03
Journal Detail:
Title:  Toxicology     Volume:  252     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2009-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
Department of Toxicology, School of Public Health, Peking University, PR China.
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MeSH Terms
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / toxicity*
Apoptosis / drug effects
Blotting, Western
Bronchi / cytology*,  drug effects
Cell Cycle / drug effects
Cell Cycle Proteins / genetics*,  metabolism*
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Transformation, Neoplastic / genetics*,  metabolism*
Databases, Protein
Electrophoresis, Gel, Two-Dimensional
Epithelial Cells / drug effects*
Gene Expression / drug effects
Isoelectric Focusing
Microtubule-Associated Proteins / genetics*,  metabolism*
Mutagens / toxicity*
Nuclear Proteins / genetics*,  metabolism*
Phosphorylation / drug effects
RNA Interference
RNA, Small Interfering
Sister Chromatid Exchange / drug effects
Tyrosine / metabolism
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Microtubule-Associated Proteins; 0/Mutagens; 0/Nuclear Proteins; 0/RNA, Small Interfering; 0/TPX2 protein, human; 55097-80-8/7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; 55520-40-6/Tyrosine

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