| TPX2 in malignantly transformed human bronchial epithelial cells by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide. | |
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MedLine Citation:
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PMID: 18723071 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to elucidate the function of the targeting protein for Xenopus kinesin-like protein 2 (Xklp2) (TPX2) in the malignant transformation of human bronchial epithelial cells induced by anti-benzo[a]pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide (anti-BPDE), TPX2 was characterized in cells at both the gene and the protein levels. TPX2 was present at higher levels in 16HBE-C cells than in 16HBE cells as demonstrated by two-dimensional gel electrophoresis, immunocytochemistry, Western blot analysis and RT-PCR. TPX2 was also detected in lung squamous-cell carcinoma tissues by immunohistochemistry, but not in normal lung tissues. Depression of TPX2 by RNA interference in 16HBE-C cells led to a decrease in cell proliferation, S-phase cell cycle arrest and cell apoptosis. Abnormal TPX2 tyrosine phosphorylation was detected in 16HBE-C cells, and this could be inhibited, to different degrees, by tyrosine kinase inhibitors. Inhibiting tyrosine phosphorylation in 16HBE-C cells by three selected tyrosine protein kinase inhibitors, tyrphostin 47, AG112 and AG555, caused G(0)/G(1)-phase cell cycle arrest. Our results suggest that anti-BPDE can cause the over-expression of TPX2 and its aberrant tyrosine phosphorylation. Misregulation of TPX2 affects the cell cycle state, proliferation rates and apoptosis. |
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Authors:
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Lijuan Zhang; He Huang; Luyao Deng; Ming Chu; Lan Xu; Juanling Fu; Yunlan Zhu; Xiuchun Zhang; Shulin Liu; Zongcan Zhou; Yuedan Wang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-03 |
Journal Detail:
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Title: Toxicology Volume: 252 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-13 Completed Date: 2009-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
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Languages: eng Pagination: 49-55 Citation Subset: IM |
Affiliation:
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Department of Toxicology, School of Public Health, Peking University, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
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toxicity* Apoptosis / drug effects Blotting, Western Bronchi / cytology*, drug effects Cell Cycle / drug effects Cell Cycle Proteins / genetics*, metabolism* Cell Line, Tumor Cell Proliferation / drug effects Cell Transformation, Neoplastic / genetics*, metabolism* Databases, Protein Electrophoresis, Gel, Two-Dimensional Epithelial Cells / drug effects* Gene Expression / drug effects Humans Immunohistochemistry Immunoprecipitation Isoelectric Focusing Microtubule-Associated Proteins / genetics*, metabolism* Mutagens / toxicity* Nuclear Proteins / genetics*, metabolism* Phosphorylation / drug effects RNA Interference RNA, Small Interfering Sister Chromatid Exchange / drug effects Tyrosine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Microtubule-Associated Proteins; 0/Mutagens; 0/Nuclear Proteins; 0/RNA, Small Interfering; 0/TPX2 protein, human; 55097-80-8/7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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