| TPCK-induced apoptosis and labelling of the largest subunit of RNA polymerase II in Jurkat cells. | |
| | |
MedLine Citation:
|
PMID: 19653099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
N-alpha-Tosyl-L: -phenylalanyl chloromethyl ketone (TPCK) is an affinity label for chymotrypsin-like proteases and has been extensively used as an experimental tool in apoptosis research to probe the role of proteases in cell death. While TPCK blocks some apoptotic changes and induces others, the cellular target or targets for TPCK have not been identified. Here we investigated for the first time the cellular targets for TPCK using a polyclonal anti-tosyl antibody. We have found that TPCK rapidly and irreversibly labels numerous intracellular proteins and have identified one as RPB1, the largest subunit of RNA polymerase II. We show that TPCK inhibits DNA binding by RNA polymerase and that TPCK inhibits transcription. Inhibition of transcription is known to induce apoptosis and while TPCK may trigger death through interaction with multiple targets, our data suggests that the pro-apoptotic effects of TPCK may be explained in part by the inhibition of RNA polymerase II activity. |
| | |
Authors:
|
Z Fabian; P O'Brien; K Pajecka; H O Fearnhead |
Related Documents
:
|
19410639 - Pro-apoptotic effects of nivalenol and deoxynivalenol trichothecenes in j774a.1 murine ... 22577489 - Flavonoids inhibit the respiratory burst of neutrophils in mammals. 12530089 - Induction of apoptosis by the green tea flavonol (-)-epigallocatechin-3-gallate in huma... 19561399 - Alpha-tocopheryl succinate potentiates the paclitaxel-induced apoptosis through enforce... 17114439 - Cyclosporin a abolishes cd28-mediated resistance to cd95-induced apoptosis via superind... 20640889 - Caspase-3 triggers a tpck-sensitive protease pathway leading to degradation of the bh3-... 7558299 - Evidence for apoptosis of murine macrophages by actinobacillus actinomycetemcomitans in... 3003359 - Basic physiology of follicular maturation in the pig. 20434119 - Changing cytokine patterns in systemic lupus: a prospective longitudinal study. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Apoptosis : an international journal on programmed cell death Volume: 14 ISSN: 1573-675X ISO Abbreviation: Apoptosis Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-04 Completed Date: 2010-01-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
|
Languages: eng Pagination: 1154-64 Citation Subset: IM |
Affiliation:
|
National Centre for Biomedical Engineering Science, School of Science, NUI Galway, Galway, Ireland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkylation
/
drug effects Antibodies / pharmacology Apoptosis / drug effects* Caspase 3 / metabolism DNA / metabolism DNA Fragmentation / drug effects Deoxyribonucleases / metabolism Enzyme Activation / drug effects Humans Jurkat Cells Protein Binding / drug effects Proteins / metabolism RNA Polymerase II / metabolism* Staining and Labeling* Tosylphenylalanyl Chloromethyl Ketone / chemistry, pharmacology* Transcription, Genetic / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies; 0/DNA fragmentation factor, human; 0/Proteins; 402-71-1/Tosylphenylalanyl Chloromethyl Ketone; 9007-49-2/DNA; EC 2.7.7.-/RNA Polymerase II; EC 2.7.7.-/RNA polymerase II largest subunit; EC 3.1.-/DFFB protein, human; EC 3.1.-/Deoxyribonucleases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Environmental-endocrine control of reproductive maturation in gastropods: implications for the mecha...
Next Document: Downregulation of uridine-cytidine kinase like-1 decreases proliferation and enhances tumor suscepti...