Document Detail

TPA-induced multinucleation of a mesenchymal stem cell-like clone is mediated primarily by karyokinesis without cytokinesis, although cell-cell fusion also occurs.
MedLine Citation:
PMID:  17599648     Owner:  NLM     Status:  MEDLINE    
The 5F9A cell, which is a mesenchymal stem cell-like clone established from rat bone marrow substrate adherent cells, can differentiate into adipocytes and osteoblasts in vitro under the appropriate conditions. Multinucleated cells could be also induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) in 5F9A cells. This effect was mediated by protein kinase C. Possible mechanisms of multinucleation by TPA were hypothesized to be either karyokinesis without cytokinesis or cell-cell fusion. By observation using time-lapse phase-contrast microscopy, we determined that the multinucleated cells were generated mainly by karyokinesis without cytokinesis. Cell fusion was studied using time-lapse photography, and confocal laser scanning microscopy using two differentially labeled cells. These techniques demonstrated that multinucleated 5F9A cells could be produced by cell fusion, albeit at a low frequency. We conclude that multinucleated 5F9A cells are formed primarily by karyokinesis without cytokinesis, although some cells are also formed by cell-cell fusion.
Keiichiro Yoshida; Shuichi Obata; Michio Ono; Michiyo Esaki; Tatsuo Maejima; Hajime Sawada
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Publication Detail:
Type:  Journal Article     Date:  2007-06-27
Journal Detail:
Title:  European journal of cell biology     Volume:  86     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-09-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  461-71     Citation Subset:  IM    
Department of Histology and Cell Biology, Yokohama City University School of Medicine, Fukuura 3-9, Kanazawa-ku, Yokohama, Kanagawa-ken 236-0004, Japan.
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MeSH Terms
Cell Adhesion / drug effects
Cell Fusion
Cell Nucleus Division / drug effects*
Clone Cells
Cytokinesis / drug effects*
DNA / analysis
Giant Cells / cytology*,  drug effects*
Laser Scanning Cytometry
Mesenchymal Stem Cells / cytology*,  drug effects*
Protein Kinase C / metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 9007-49-2/DNA; EC Kinase C

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