Document Detail


LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-box protein involved in light-dependent development and gene expression, undergoes COP1-mediated ubiquitination.
MedLine Citation:
PMID:  18796637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B-box containing proteins play an important role in light signaling in plants. Here, we identify LIGHT-REGULATED ZINC FINGER1/SALT TOLERANCE HOMOLOG3 (STH3), a B-box encoding gene that genetically interacts with two key regulators of light signaling, ELONGATED HYPOCOTYL5 (HY5) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). STH3 physically interacts with HY5 in vivo and shows a COP1-dependent localization to nuclear speckles when coexpressed with COP1 in plant cells. A T-DNA insertion mutant, sth3, is hyposensitive to high fluence blue, red, and far-red light and has elongated hypocotyls under short days. Analyses of double mutants between sth3, sth2, and hy5 suggest that they have partially overlapping functions. Interestingly, functional assays in protoplasts suggest that STH3 can activate transcription both independently and together with STH2 through the G-box promoter element. Furthermore, sth3 suppresses the cop1 hypocotyl phenotype in the dark as well as the anthocyanin accumulation in the light. Finally, COP1 ubiquitinates STH3 in vitro, suggesting that STH3 is regulated by COP1. In conclusion, we have identified STH3 as a positive regulator of photomorphogenesis acting in concert with STH2 and HY5, while also being a target of COP1-mediated ubiquitination.
Authors:
Sourav Datta; Henrik Johansson; Chamari Hettiarachchi; María Luisa Irigoyen; Mintu Desai; Vicente Rubio; Magnus Holm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-16
Journal Detail:
Title:  The Plant cell     Volume:  20     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2009-04-15     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2324-38     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology, Gothenburg University, 405 30 Gothenburg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / genetics*,  growth & development,  metabolism
Arabidopsis Proteins / genetics*,  metabolism
Basic-Leucine Zipper Transcription Factors / genetics,  metabolism
Gene Expression Regulation, Developmental / radiation effects*
Gene Expression Regulation, Plant / radiation effects*
Light*
Molecular Sequence Data
Nuclear Proteins / genetics,  metabolism
Protein Binding / radiation effects
Sequence Homology, Amino Acid
Transcription Factors / genetics,  metabolism
Two-Hybrid System Techniques
Ubiquitination / radiation effects
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Basic-Leucine Zipper Transcription Factors; 0/HY5 protein, Arabidopsis; 0/LZF1 protein, Arabidopsis; 0/Nuclear Proteins; 0/Transcription Factors; 0/constitutive photomorphogenic 1 protein, Arabidopsis
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