| TNFR gene-modified mesenchymal stem cells attenuate inflammation and cardiac dysfunction following MI. | |
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MedLine Citation:
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PMID: 17852784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To investigate the protective effect of tumor necrosis factor receptor (TNFR) gene modified mesenchymal stem cells (MSCs) transplantation against inflammation and cardiac dysfunction following acute myocardial infarction (AMI). DESIGN: MSCs were extracted from the tibias and femurs of rats and transfected with recombinant adeno-associated viral (rAAV) expressing EGFP (enhanced green fluorescent protein) or p75 (human 75 kilodalton) TNFR at multiplicity of infection of 10(5) particles/cell. Rats with AMI induced by occlusion of the left coronary artery were randomized to MSCs-TNFR transplantation group, MSCs-EGFP transplantation group and MI control group. RESULTS: The effects of MSCs-TNFR transplantation on cardiac inflammation and left ventricular dysfunction were observed after 2 weeks of MI. We found that: 1) MSCs-TNFR transplantation attenuated protein production and gene expression of inflammatory cytokines TNF-, IL-1beta and IL-6; 2) MSCs-TNFR transplantation inhibited cardiomyocytes apoptosis and 3) MSCs-TNFR transplantation improved left ventricular function. CONCLUSIONS: The experimental data show that transplantation with rAAV-TNFR transfected MSCs improves left ventricular function following MI through anti-apoptotic and anti-inflammatory mechanisms. |
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Authors:
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Cuiyu Bao; Jun Guo; Guosheng Lin; Mingyan Hu; Zhimin Hu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian cardiovascular journal : SCJ Volume: 42 ISSN: 1401-7431 ISO Abbreviation: Scand. Cardiovasc. J. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-05-06 Completed Date: 2008-07-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708377 Medline TA: Scand Cardiovasc J Country: England |
Other Details:
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Languages: eng Pagination: 56-62 Citation Subset: IM |
Affiliation:
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Cardiovascular Research Institute, Xianning College, and Department of Cardiology, Renmin Hospital, Hubei, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Cells, Cultured Dependovirus / genetics Disease Models, Animal Down-Regulation Gene Therapy / methods* Genetic Vectors Humans Inflammation Mediators / metabolism Interleukin-1beta / metabolism Interleukin-6 / metabolism Male Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells / metabolism* Myocardial Infarction / complications, genetics, metabolism, surgery, therapy* Myocarditis / etiology, genetics, metabolism, prevention & control* Myocytes, Cardiac / metabolism*, pathology Rats Rats, Sprague-Dawley Receptors, Tumor Necrosis Factor, Type II / genetics, metabolism* Time Factors Transfection Tumor Necrosis Factor-alpha / metabolism Ventricular Dysfunction, Left / etiology, genetics, metabolism, prevention & control* |
| Chemical | |
Reg. No./Substance:
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0/Inflammation Mediators; 0/Interleukin-1beta; 0/Interleukin-6; 0/Receptors, Tumor Necrosis Factor, Type II; 0/TNFRSF1B protein, human; 0/Tumor Necrosis Factor-alpha |
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