Document Detail


TNF, but not IL-6 and IL-17, is crucial for the development of T cell-independent psoriasis-like dermatitis in Il1rn-/- mice.
MedLine Citation:
PMID:  20610641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IL-1 is a proinflammatory cytokine consisting of two molecular species, IL-1alpha and IL-1beta, and IL-1R antagonist (gene: Il1rn) is the endogenous suppressor. Il1rn(-/-) mice spontaneously develop autoimmune diseases, such as arthritis and aortitis, and a dermatitis that histologically resembles human psoriasis. The pathogenic mechanisms underlying this dermatitis, however, remain to be elucidated. In this study, we demonstrated that the production of inflammatory cytokines and chemokines was enhanced at the site of inflammation. The development of dermatitis was completely suppressed in Tnfsf1a(-/-) but not in Il6(-/-) mice, similar to that observed in arthritis and aortitis. However, IL-17 deficiency did not affect the development of dermatitis at all, in clear contrast to that of arthritis and aortitis. Different from arthritis and aortitis, adoptive transfer of Il1rn(-/-) T cells did not induce dermatitis in the recipient SCID mice and skin lesions developed in Il1rn(-/-) SCID mice, indicating that T cells are not involved in the development of skin lesions. In support for this, bone marrow cell transplantation experiments showed that TNF produced by skin residential cells, but not bone marrow cell-derived cells, was important for the development of dermatitis. Furthermore, we showed that IL-1 directly enhanced TNF and chemokine expression in keratinocytes. These observations suggest that excess IL-1 signaling directly activates keratinocytes to produce TNF and chemokines, resulting in the development of psoriasis-like skin lesions without the involvement of autoimmunity in Il1rn(-/-) mice.
Authors:
Akiko Nakajima; Taizo Matsuki; Mayumi Komine; Akihiko Asahina; Reiko Horai; Susumu Nakae; Harumichi Ishigame; Shigeru Kakuta; Shinobu Saijo; Yoichiro Iwakura
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-07
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  185     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-27     Completed Date:  2010-09-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1887-93     Citation Subset:  AIM; IM    
Affiliation:
Center for Experimental Medicine and Systems Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dermatitis, Contact / immunology*,  metabolism,  pathology
Female
Inflammation Mediators / metabolism,  physiology
Interleukin 1 Receptor Antagonist Protein / deficiency*,  genetics*
Interleukin-17 / deficiency,  genetics,  physiology*
Interleukin-6 / deficiency,  genetics,  physiology*
Mice
Mice, Inbred BALB C
Mice, Knockout
Mice, SCID
Psoriasis / immunology*,  metabolism,  pathology
Skin / immunology,  metabolism,  pathology
T-Lymphocyte Subsets / immunology*,  pathology,  transplantation
Tumor Necrosis Factor-alpha / biosynthesis,  physiology*
Chemical
Reg. No./Substance:
0/Il1rn protein, mouse; 0/Inflammation Mediators; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-17; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha

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