Document Detail


TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food.
MedLine Citation:
PMID:  22778626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcus aureus is a major bacterial pathogen which causes clinical infections and food poisoning. This bacterium produces a group of twenty-one enterotoxins (SEs). These enterotoxins have two separate but related biological activities. They cause gastroenteritis and function as superantigens that activate large numbers of T cells. The current method for detection of enterotoxins activity is an in vivo monkey or kitten bioassay; however, this method is not practical to test on a large number of samples. Several immunological assays have been developed however, but these assays cannot distinguish between active toxin which causes food poisoning and inactive toxin, which can bind antibody, but shows no toxicity. The current study demonstrates that short term ex vivo exposure of primary naïve CD4(+) T-cells or splenocytes to SEA induces differential expression and secretion of tumor necrosis factor (TNF) protein. We used immunomagnetic beads coated with anti-SEA antibody to specifically isolate SEA from food. After the eluted toxin was added to the cells SEA biological activity was measured by quantifying TNF protein expression or secretion.
Authors:
Reuven Rasooly; Bradley Hernlem
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2012-05-10
Journal Detail:
Title:  Sensors (Basel, Switzerland)     Volume:  12     ISSN:  1424-8220     ISO Abbreviation:  Sensors (Basel)     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-10     Completed Date:  2012-11-01     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101204366     Medline TA:  Sensors (Basel)     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  5978-85     Citation Subset:  IM    
Affiliation:
Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA. reuven.rasooly@ars.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis*
CD4-Positive T-Lymphocytes / drug effects
Enterotoxins / analysis*,  toxicity
Enzyme-Linked Immunosorbent Assay
Female
Food Contamination / analysis*
Mice
Mice, Inbred C57BL
Tumor Necrosis Factor-alpha / analysis*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Enterotoxins; 0/Tumor Necrosis Factor-alpha; 37337-57-8/enterotoxin A, Staphylococcal
Comments/Corrections

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