Document Detail

TNF-alpha stimulates alveolar liquid clearance during intestinal ischemia-reperfusion in rats.
MedLine Citation:
PMID:  10645884     Owner:  NLM     Status:  MEDLINE    
Intestinal ischemia-reperfusion commonly occurs in critically ill patients and may lead to the development of remote organ injury, frequently involving the lungs. In the present study, alveolar liquid clearance was studied in ventilated, anesthetized rats subjected to 45 min of intestinal ischemia followed by 3 h of reperfusion. An isosmolar 5% albumin solution was instilled into the lungs, and alveolar liquid clearance was measured from the increase in alveolar protein concentration as water was reabsorbed over 45 min. Intestinal ischemia-reperfusion resulted in a 76% increase in alveolar liquid clearance compared with the control value (P < 0.05). The stimulated alveolar liquid clearance seen after intestinal ischemia-reperfusion was not inhibited by propranolol, indicating stimulation through a noncatecholamine-dependent pathway. Intestinal ischemia-reperfusion did not result in increased intracellular cAMP levels. Amiloride inhibited similar fractions in animals subjected to ischemia-reperfusion and control animals. Administration of a neutralizing polyclonal anti-tumor necrosis factor-alpha antibody before induction of intestinal ischemia completely inhibited the increased alveolar liquid clearance observed after intestinal ischemia-reperfusion. In conclusion, our results suggest that intestinal ischemia-reperfusion in rats leads to stimulation of alveolar liquid clearance and that this stimulation is mediated, at least in part, by a tumor necrosis factor-alpha-dependent mechanism.
A Börjesson; A Norlin; X Wang; R Andersson; H G Folkesson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  278     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-31     Completed Date:  2000-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L3-12     Citation Subset:  IM    
Department of Surgery, Lund University Hospital, SE-221 85 Lund, Sweden.
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MeSH Terms
Adrenergic beta-Antagonists / pharmacology
Amiloride / pharmacology
Antibodies, Monoclonal / immunology,  pharmacology
Body Water / metabolism
Capillary Permeability / drug effects
Cyclic AMP / biosynthesis
Endothelium, Vascular / metabolism
Intestines / blood supply*
Ischemia / metabolism*
Lung / metabolism
Propranolol / pharmacology
Proteins / metabolism
Pulmonary Alveoli / drug effects,  metabolism*
Rats, Sprague-Dawley
Reference Values
Reperfusion Injury / metabolism*
Tumor Necrosis Factor-alpha / analysis,  immunology,  pharmacology*
Reg. No./Substance:
0/Adrenergic beta-Antagonists; 0/Antibodies, Monoclonal; 0/Proteins; 0/Tumor Necrosis Factor-alpha; 2609-46-3/Amiloride; 525-66-6/Propranolol; 60-92-4/Cyclic AMP

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