| TNF alpha is localized to nasal mucosal mast cells and is released in acute allergic rhinitis. | |
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MedLine Citation:
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PMID: 7553243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Allergic mucosal inflammation is characterized by tissue infiltration with eosinophils, and associated activation of mast cells and T lymphocytes. Tumour necrosis factor (TNF) alpha/cachectin is a candidate cytokine relevant to the pathogenesis of these events through its capacity to upregulate the expression of endothelial cell adhesion molecules, mediate granulocyte chemoattraction, and activate eosinophils, mast cells and T cells. To investigate the presence and localization of TNF alpha in the nasal mucosa in allergic rhinitis, nasal biopsies from perennial rhinitic (n = 13) and non-rhinitic volunteers (n = 11) were embedded in glycol methacrylate and immunostained with a monoclonal antibody directed against TNF alpha, and adjacent 2 microns sections stained for tryptase, CD3 and eosinophil cationic protein. This identified positive immunostaining for TNF alpha in the submucosa of both the rhinitic and normal subjects (median cell counts 13 and 23 cells/mm2 respectively, P = 0.24) with cellular localization to mast cells but not to T-lymphocytes or eosinophils. In a subsequent study of seven atopic subjects, nasal allergen challenge produced increases in lavage levels of histamine and albumin, which was associated with significant release of TNF alpha as early as 2 min post-allergen when compared with the saline control day (P = 0.05). This difference was also apparent when studying the area under the curve both at 30 and 60 min post-challenge t-test (P = 0.015 and 0.02 respectively).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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P Bradding; R Mediwake; I H Feather; J Madden; M K Church; S T Holgate; P H Howarth |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 25 ISSN: 0954-7894 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-11-09 Completed Date: 1995-11-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 406-15 Citation Subset: IM |
Affiliation:
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Immunopharmacology Group, Southampton University, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Albumins / metabolism Biopsy Chymases Eosinophils / immunology Female Histamine / metabolism Humans Immunohistochemistry Inflammation Mediators / pharmacology Male Mast Cells / immunology, metabolism*, secretion* Nasal Lavage Fluid / chemistry Nasal Mucosa / immunology, metabolism*, secretion* Rhinitis, Allergic, Perennial / immunology, metabolism*, physiopathology Serine Endopeptidases / metabolism Tryptases Tumor Necrosis Factor-alpha / immunology, metabolism*, secretion* |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Inflammation Mediators; 0/Tumor Necrosis Factor-alpha; 51-45-6/Histamine; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/chymase 2; EC 3.4.21.39/Chymases; EC 3.4.21.59/Tryptases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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