| TNF-alpha from monocyte of patients with pre-eclampsia-induced apoptosis in human trophoblast cell line. | |
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MedLine Citation:
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PMID: 17688605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: In pre-eclampsia, fetal growth restriction is frequently observed, and the possible involvement of inhibitory substances on trophoblast cell proliferation and differentiation has been suggested. The objective of this study was to investigate the effects humoral factors, such as cytokines, produced in immune cells on proliferation of an immortalized trophoblastic cell line (TCL) that we established. METHODS: Serum and lymphocyte layers were isolated from the blood of normal pregnant and preeclamptic women. The lymphocyte layer was further fractionated into different immune cell populations by the Stem Sep method. Immortalized trophoblastic cells were cultured with the sera diluted. The cytokine concentrations in the supernatants of lymphocyte cultures were compared between normal pregnancy and pre-eclampsia. The number, DNA content and induced apoptosis were examined on the immortalized trophoblastic cells at the end of culture. RESULTS: The sera from preeclamptic women significantly inhibited the immortalized trophoblastic cell proliferation in comparison with those from normal pregnancy. Among the lymphocyte fractions, monocytes significantly inhibited the immortalized trophoblastic cell proliferation. The monocytes from preeclamptic women were found to produce higher levels of tumor necrosis factor-alpha (TNF-alpha) in the culture supernatant than those from normal pregnant women. The coculture with the monocytes from preeclamptic women increased the frequency of TUNEL-positive TCL cells. TNF-alpha inhibited immortalized trophoblastic cell proliferation in a dose-dependent manner and induced apoptosis. CONCLUSION: The present results suggest that monocytes are activated and that cytokines, such as TNF-alpha, which is produced by monocytes, induce apoptosis and inhibit proliferation of trophoblast cells in pre-eclampsia. |
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Authors:
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Hiroyuki Seki; Kikumi Matuoka; Hiroki Inooku; Satoru Takeda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of obstetrics and gynaecology research Volume: 33 ISSN: 1341-8076 ISO Abbreviation: J. Obstet. Gynaecol. Res. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-08-10 Completed Date: 2007-10-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9612761 Medline TA: J Obstet Gynaecol Res Country: Japan |
Other Details:
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Languages: eng Pagination: 408-16 Citation Subset: IM |
Affiliation:
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Saitama Medical Center, Saitama Medical University, Obstetrics and Gynecology, Kawagoe, Japan. h-n-seki@theia.ocn.ne.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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immunology* Coculture Techniques Female Humans In Situ Nick-End Labeling Monocytes / immunology*, pathology Pre-Eclampsia / blood*, immunology, pathology Pregnancy Trophoblasts / cytology, drug effects, immunology* Tumor Necrosis Factor-alpha / biosynthesis, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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