Document Detail


TNF-Related Apoptosis-Inducing Ligand TRAIL as a Potential Biomarker for Early Pregnancy Complications.
MedLine Citation:
PMID:  22419717     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Context:Recurrent miscarriage (RM) (at least three consecutive pregnancy losses) occurs in 1-3% of fertile couples. No biomarkers with high predictive value of threatening miscarriage have been identified.Objective:We aimed to profile whole-genome differential gene expression in RM placental tissue and to determine the protein levels of identified loci in maternal sera in early pregnancy.Design and Subjects:GeneChips (Affymetrix) were used for discovery and TaqMan RT-quantitative PCR assays for replication of mRNA expression in placentas from RM cases (n = 13) compared with uncomplicated pregnancies matched for gestational age (n = 23). Concentrations of soluble TRAIL (sTRAIL) and calprotectin in maternal serum in normal first-trimester (n=35) and failed pregnancies (early miscarriage, n = 18, late miscarriage, n = 4; tubal pregnancy, n = 11) were determined using ELISA.Results:In RM placentas, 30 differentially expressed (P < 0.05, false discovery rate adjusted) transcripts were identified. Significantly increased placental mRNA expression of TNF-related apoptosis-inducing ligand (TRAIL; P = 1.4 × 10(-3); 1.68-fold change) and S100A8 (P = 7.9 × 10(-4); 2.56-fold change) encoding for inflammatory marker calprotectin (S100A8/A9) was confirmed by RT-quantitative PCR. When compared with normal first-trimester pregnancy (sTRAIL 16.1 ± 1.6 pg/ml), significantly higher maternal serum concentration of sTRAIL was detected at the RM event (33.6 ± 4.3 pg/ml, P = 0.00027) and in pregnant women who developed an unpredicted miscarriage 2-50 d after prospective serum sampling (28.5 ± 4.4 pg/ml, P = 0.039). Women with tubal pregnancy also exhibited elevated sTRAIL (30.5 ± 3.9 pg/ml, P = 0.035). Maternal serum levels of calprotectin were neither diagnostic nor prognostic to early pregnancy failures (P > 0.05).Conclusion:The study indicated sTRAIL as a potential predictive biomarker in maternal serum for early pregnancy complications.
Authors:
Kristiina Rull; Kärt Tomberg; Sulev Kõks; Jaana Männik; Märt Möls; Meeli Sirotkina; Signe Värv; Maris Laan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-14
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  -     ISSN:  1945-7197     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Human Molecular Genetics Group (K.R., K.T., J.M., M.L.) and Department of Cell Biology (S.V.), Institute of Molecular and Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu 51010, Estonia; Department of Physiology (S.K.), University of Tartu, Tartu 50090, Estonia; Institute of Mathematical Statistics (M.M.), University of Tartu, Tartu 50409, Estonia; and Department of Obstetrics and Gynecology (K.R.) and Department of Pathology (M.S.), Tartu University Hospital, Tartu 51014, Estonia.
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