Document Detail


TLR9 is expressed in idiopathic interstitial pneumonia and its activation promotes in vitro myofibroblast differentiation.
MedLine Citation:
PMID:  18633634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infectious diseases can be cofactors in idiopathic interstitial pneumonias (IIP) pathogenesis; recent data suggests that toll-like receptors 9 (TLR9) ligands contribute to experimental chronic tissue remodeling. Real-time TAQMAN and immunohistochemical analysis of IIP normal surgical lung biopsies (SLBs), primary fibroblast lines grown from both IIP and normal SLBs indicate that TLR9 is prominently and differentially expressed in a disease-specific manner. TLR9 expression was increased in biopsies from patients with IIP compared with normal lung biopsies and its expression is localized to areas of marked interstitial fibrosis. TLR9 in fibroblasts appeared to be increased by profibrotic Th2 cytokines (IL-4 and IL-13) and this was true in fibroblasts cultured from the most severe form of IIP, idiopathic pulmonary fibrosis (IPF) SLBs, in non-specific interstitial pneumonia fibroblast lines, and in normal fibroblasts. Finally, confocal microscopy studies have shown that TLR9 activation by its synthetic agonist CpG-ODN significantly increased the expression of alpha smooth muscle actin, the main marker of myofibroblast differentiation. These data indicate that TLR9 expression may drive the abnormal tissue healing response in severe forms of IIP and its activation can have a key role in myofibroblast differentiation promoting the progression of disease during the terminal phase of IPF.
Authors:
A Meneghin; E S Choi; H L Evanoff; S L Kunkel; F J Martinez; K R Flaherty; G B Toews; C M Hogaboam
Related Documents :
16299104 - The expression of the pituitary growth hormone-releasing hormone receptor and its splic...
17003084 - Induction of surfactant protein a expression by cortisol facilitates prostaglandin synt...
18070924 - Abnormal heart development and lung remodeling in mice lacking the hypoxia-inducible fa...
12827614 - Thyroid transcription factor-1 expression prevalence and its clinical implications in n...
10934014 - Decapentaplegic is a direct target of dtcf repression in the drosophila visceral mesoderm.
21257914 - Pulsatile to-fro flow induces greater and sustained expression of tissue factor rna in ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-07-17
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  130     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2008-12-11     Revised Date:  2010-12-03    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  979-92     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Michigan Medical School, Room 4710, BSRB, 109 Zina Pitcher Pl, Ann Arbor, MI 48109-2200, USA. alessiam@med.umich.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Cell Differentiation* / drug effects
Cells, Cultured
Fibroblasts / drug effects,  immunology*,  pathology
Humans
Idiopathic Interstitial Pneumonias / genetics,  immunology*,  pathology
Idiopathic Pulmonary Fibrosis / genetics,  immunology*,  pathology
Immunohistochemistry
Interleukin-13 / metabolism
Interleukin-4 / metabolism
Lung / drug effects,  immunology*,  pathology
Microscopy, Confocal
Oligodeoxyribonucleotides / pharmacology
Polymerase Chain Reaction
RNA, Messenger / metabolism
Recombinant Proteins / metabolism
Toll-Like Receptor 9 / agonists,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
P50 HL056402-019001/HL/NHLBI NIH HHS; P50 HL056402-029001/HL/NHLBI NIH HHS; P50 HL056402-039001/HL/NHLBI NIH HHS; P50 HL056402-049001/HL/NHLBI NIH HHS; P50 HL056402-059001/HL/NHLBI NIH HHS; P50 HL056402-069001/HL/NHLBI NIH HHS; P50 HL056402-079001/HL/NHLBI NIH HHS; P50 HL056402-089001/HL/NHLBI NIH HHS; P50 HL056402-099001/HL/NHLBI NIH HHS; P50 HL056402-109001/HL/NHLBI NIH HHS; P50 HL56402/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/CPG-oligonucleotide; 0/Interleukin-13; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 0/Recombinant Proteins; 0/TLR9 protein, human; 0/Toll-Like Receptor 9; 207137-56-2/Interleukin-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hepatic gap junctions in the hepatocarcinogen-resistant DRH rat.
Next Document:  Measurements of clothing insulation with a thermal manikin operating under the thermal comfort regul...