| TLR4 recognizes Pseudallescheria boydii conidia and purified rhamnomannans. | |
| | |
MedLine Citation:
|
PMID: 20959459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pseudallescheria boydii (Scedosporium apiospermum) is a saprophytic fungus widespread in the environment, and has recently emerged as an agent of localized as well as disseminated infections, particularly mycetoma, in immunocompromised and immunocompetent hosts. We have previously shown that highly purified α-glucan from P. boydii activates macrophages through Toll-like receptor TLR2, however, the mechanism of P. boydii recognition by macrophage is largely unknown. In this work, we investigated the role of innate immune receptors in the recognition of P. boydii. Macrophages responded to P. boydii conidia and hyphae with secretion of proinflammatory cytokines. The activation of macrophages by P. boydii conidia required functional MyD88, TLR4, and CD14, whereas stimulation by hyphae was independent of TLR4 and TLR2 signaling. Removal of peptidorhamnomannans from P. boydii conidia abolished induction of cytokines by macrophages. A fraction highly enriched in rhamnomannans was obtained and characterized by NMR, high performance TLC, and GC-MS. Preparation of rhamnomannans derived from P. boydii triggered cytokine release by macrophages, as well as MAPKs phosphorylation and IκBα degradation. Cytokine release induced by P. boydii-derived rhamnomannans was dependent on TLR4 recognition and required the presence of non-reducing end units of rhamnose of the rhamnomannan, but not O-linked oligosaccharides from the peptidorhamnomannan. These results imply that TLR4 recognizes P. boydii conidia and this recognition is at least in part due to rhamnomannans expressed on the surface of P. boydii. |
| | |
Authors:
|
Rodrigo T Figueiredo; Patrícia L Fernandez; Fabianno F Dutra; Yissett González; Lívia Cristina Lopes; Vera Carolina B Bittencourt; Guilherme L Sassaki; Eliana Barreto-Bergter; Marcelo T Bozza |
Related Documents
:
|
17885949 - Immune response to candida albicans is preserved despite defect in o-mannosylation of s... 22119009 - Probing cytokines, chemokines and matrix metalloproteinases towards better immunotherap... 18375859 - Cd14 and tlr4 are expressed early in tammar (macropus eugenii) neonate development. 16428799 - Upregulation of immunoregulatory src homology 2 molecule containing inositol phosphatas... 17085599 - Dual recognition of herpes simplex viruses by tlr2 and tlr9 in dendritic cells. 18779779 - Critical role of the nadph oxidase subunit p47phox on vascular tlr expression and neoin... 20570159 - 16-bromoepiandrosterone, an activator of the mammalian immune system, inhibits glucose ... 23449239 - The immune response to one-lung-ventilation is not affected by repeated alveolar recrui... 10858959 - Apoptosis and cell cycle: distinct checkpoints with overlapping upstream control. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-19 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-20 Completed Date: 2011-01-24 Revised Date: 2011-12-26 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 40714-23 Citation Subset: IM |
Affiliation:
|
Departamento de Imunologia, Laboratório de Inflamação e Imunidade, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 941-902 Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD14 / immunology, metabolism Cytokines / immunology, secretion Hyphae / immunology, metabolism Immunity, Innate / immunology Macrophage Activation / immunology* Macrophages, Peritoneal / immunology*, metabolism Mannans / immunology*, metabolism Mice Mice, Knockout Mitogen-Activated Protein Kinase Kinases / immunology, metabolism Myeloid Differentiation Factor 88 / immunology, metabolism Pseudallescheria / immunology*, metabolism Spores, Fungal / immunology*, metabolism Toll-Like Receptor 2 / immunology, metabolism Toll-Like Receptor 4 / immunology*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD14; 0/Cytokines; 0/Mannans; 0/Myd88 protein, mouse; 0/Myeloid Differentiation Factor 88; 0/Tlr2 protein, mouse; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A conserved protein interaction interface on the type 5 G protein beta subunit controls proteolytic ...
Next Document: Leukotriene B4 augments and restores Fc gammaRs-dependent phagocytosis in macrophages.