| TLR Agonists Downregulate H2-O in CD8{alpha}- Dendritic Cells. | |
| | |
MedLine Citation:
|
PMID: 21918198 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Peptide loading of MHC class II (MHCII) molecules is catalyzed by the nonclassical MHCII-related molecule H2-M. H2-O, another MHCII-like molecule, associates with H2-M and modulates H2-M function. The MHCII presentation pathway is tightly regulated in dendritic cells (DCs), yet how the key modulators of MHCII presentation, H2-M and H2-O, are affected in different DC subsets in response to maturation is unknown. In this study, we show that H2-O is markedly downregulated in vivo in mouse CD8α(-) DCs in response to a broad array of TLR agonists. In contrast, CD8α(+) DCs only modestly downregulated H2-O in response to TLR agonists. H2-M levels were slightly downmodulated in both CD8α(-) and CD8α(+) DCs. As a consequence, H2-M/H2-O ratios significantly increased for CD8α(-) but not for CD8α(+) DCs. The TLR-mediated downregulation was DC specific, as B cells did not show significant H2-O and H2-M downregulation. TLR4 signaling was required to mediate DC H2-O downregulation in response to LPS. Finally, our studies showed that the mechanism of H2-O downregulation was likely due to direct protein degradation of H2-O as well as downregulation of H2-O mRNA levels. The differential H2-O and H2-M modulation after DC maturation supports the proposed roles of CD8α(-) DCs in initiating CD4-restricted immune responses by optimal MHCII presentation and of CD8α(+) DCs in promoting immune tolerance via presentation of low levels of MHCII-peptide. |
| | |
Authors:
|
Gavin W Porter; Woelsung Yi; Lisa K Denzin |
Related Documents
:
|
17182568 - The critical role of protein kinase c-theta in fas/fas ligand-mediated apoptosis. 11204278 - Fas-induced apoptosis is a rare event in sjögren's syndrome. 16273638 - Mechanism of counterattack of colorectal cancer cell by fas/fas ligand system. 9548518 - Cell death during autoimmune demyelination: effector but not target cells are eliminate... 9712368 - Infection of t cell subsets by hiv-1 and the effects of interleukin-12. 7586728 - Regulation of human alveolar macrophage inflammatory cytokines by tyloxapol: a componen... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-9-14 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: - ISSN: 1550-6606 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-9-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Immunology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10065; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-CD180 (RP105) activates B cells to rapidly produce polyclonal Ig via a T cell and MyD88-indepen...
Next Document: Collaborating with diabetes educators to promote smoking cessation for people with diabetes: the cal...