Document Detail

TIMP-1 production in human retinal pigment epithelial cells after laser exposure.
MedLine Citation:
PMID:  17162914     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate changes in the production of tissue inhibitor of metalloproteinase type 1 (TIMP-1) by human retinal pigment epithelial (RPE) cells following argon laser exposure. METHODS: Human cultured ARPE19 cells were exposed to argon green laser at four different energy levels ranging from 60mW to 360mW. After laser exposure, the culture media were sampled at 0, 24, 72 and 144 hours for TIMP-1 concentration produced by the RPE cells. The levels of TIMP-1 in the cells treated with different laser energy levels were compared with a control group not exposed to laser application. Immunocytochemistry for proliferating cell nuclear antigen (PCNA) was performed to detect any adverse effects on the RPE cells caused by laser exposure. RESULTS: Immediately after laser exposure, the concentration of TIMP-1 was not detectable. At 24 hours after laser exposure, the concentration of TIMP-1 increased significantly in RPE cells treated with 120mW and 240mW at 24 hours (P=0.006 and P=0.001 respectively) compared with control cells. At 72 hours after treatment, RPE cells treated at 120mW, 240mW and 360mW demonstrated significantly increase in TIMP-1 production compared with control (P=0.003, P < 0.001 and P < 0.001, respectively). No significant reduction in cell viability was observed following laser application as detected by PCNA expression. CONCLUSIONS: Our results demonstrated that early TIMP-1 production by RPE cells in cell cultures was enhanced following laser exposure.
Alvin K H Kwok; Timothy Y Y Lai; Hin-Fai Yam; Chi-Pui Pang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu     Volume:  21     ISSN:  1000-4432     ISO Abbreviation:  Yan Ke Xue Bao     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2006-12-13     Completed Date:  2009-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605666     Medline TA:  Yan Ke Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  31-7     Citation Subset:  IM    
Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong SAR.
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MeSH Terms
Cell Line
Pigment Epithelium of Eye / metabolism*,  radiation effects
Time Factors
Tissue Inhibitor of Metalloproteinase-1 / biosynthesis*
Reg. No./Substance:
0/Tissue Inhibitor of Metalloproteinase-1

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