Document Detail

TGF-beta1 stimulates IL-8 release, COX-2 expression, and PGE(2) release in human airway smooth muscle cells.
MedLine Citation:
PMID:  10893219     Owner:  NLM     Status:  MEDLINE    
We have recently shown that endogenous prostanoids are critical in bradykinin-stimulated interleukin (IL)-8 release from human airway smooth muscle (ASM) cells. In this study, we tested the ability of transforming growth factor (TGF)-beta1 to stimulate IL-8 release, cyclooxygenase (COX)-2 expression and PGE(2) generation in cultured human ASM cells and explored the role of COX products and COX-2 induction on IL-8 release. TGF-beta1 stimulated IL-8 release, COX-2 induction, and PGE(2) generation in a concentration- and time-dependent manner. Maximal IL-8 release was achieved with 10 ng/ml of TGF-beta1 after 16 h of incubation, which was inhibited by the transcription inhibitor actinomycin D and the corticosteroid dexamethasone but was not affected by the nonselective COX inhibitor indomethacin and the selective COX-2 inhibitor NS-398 despite their inhibition on TGF-beta1-induced PGE(2) release. These results show for the first time that TGF-beta1 stimulates IL-8 release, COX-2 induction, and PGE(2) generation in human ASM cells and that PGE(2) generation is not critical for TGF-beta1-induced IL-8 release. These findings suggest that TGF-beta1 may play an important role in the pathophysiology of asthma.
C Y Fong; L Pang; E Holland; A J Knox
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  279     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-08     Completed Date:  2000-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L201-7     Citation Subset:  IM    
Division of Respiratory Medicine, City Hospital, University of Nottingham, Nottingham NG5 1PB, United Kingdom.
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MeSH Terms
Aged, 80 and over
Cell Survival / drug effects
Cells, Cultured
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors / pharmacology
Dactinomycin / pharmacology
Dexamethasone / pharmacology
Dinoprostone / metabolism*
Enzyme Induction
Glucocorticoids / pharmacology
Interleukin-8 / metabolism*
Isoenzymes / metabolism*
Membrane Proteins
Muscle, Smooth / cytology,  metabolism*,  physiology
Prostaglandin-Endoperoxide Synthases / metabolism*
Protein Synthesis Inhibitors / pharmacology
Trachea / cytology,  metabolism*,  physiology
Transforming Growth Factor beta / pharmacology*
Reg. No./Substance:
0/Cyclooxygenase 2 Inhibitors; 0/Cyclooxygenase Inhibitors; 0/Glucocorticoids; 0/Interleukin-8; 0/Isoenzymes; 0/Membrane Proteins; 0/Protein Synthesis Inhibitors; 0/Transforming Growth Factor beta; 363-24-6/Dinoprostone; 50-02-2/Dexamethasone; 50-76-0/Dactinomycin; EC 2; EC protein, human; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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