Document Detail

TGF-beta1 and high levels of glucose do not increase insulin cell proportions in the avian embryonic pancreas.
MedLine Citation:
PMID:  18097729     Owner:  NLM     Status:  MEDLINE    
TGF-beta1 is thought to decrease the proportion of embryonic pancreatic beta-cells with respect to alpha-cells, whereas glucose is thought to enhance beta-cell proportions in rats. However, chick pancreatic cells may respond in a dissimilar way to glucose. Thus, the effect of TGF-beta1 on the proportion of beta-cells in embryonic chick dorsal pancreatic buds (DPBs) in vitro was tested with short-term exposure to high levels of glucose. Five-day-old chick DPBs were cultured on growth factor-reduced Matrigel, which contains reduced levels of growth factors including TGF-beta1, and a variety of culture media with and without high levels of glucose. TGF-beta1 reduced the proportion of beta-cells, as expected. A similar decrease in the proportion of beta-cells occurred in the presence of high levels of glucose.
A Marais; P T Goven-Shiba; B Kramer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-20
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  44     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  41-3     Citation Subset:  IM    
Embryonic Differentiation and Development Research Programme, School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa.
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MeSH Terms
Cells, Cultured
Chick Embryo
Glucose / metabolism*
Insulin / metabolism
Insulin-Secreting Cells* / cytology,  metabolism
Pancreas / cytology*,  embryology*,  metabolism
Transforming Growth Factor beta1 / metabolism*
Reg. No./Substance:
0/Transforming Growth Factor beta1; 11061-68-0/Insulin; 50-99-7/Glucose

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