| TGF-beta control of rat thyroid follicular cells differentiation. | |
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MedLine Citation:
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PMID: 12972178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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TGF-beta1 is a potent inhibitor of growth and DNA synthesis in thyroid cells. It has also been shown that TGF-beta1 inhibits thyrocyte function. The functional inhibition is represented by a downregulation of thyroid specific genes, such as Na(+)/I(-) symporter (NIS), thyroglobulin (TG) and thyroperoxidase (TPO). The transcriptional control of these genes is mediated by thyroid-specific transcription factors: thyroid transcription factor-1 (TTF-1) and PAX-8. It has been shown that Smad proteins play a pivotal role in the intracellular signal transduction of the TGF-beta family members. In this paper, the functional relevance of Smad4, in the control of thyroid differentiation genes and thyroid-specific transcription factors, has been investigated. The data obtained provides, for the first time, evidence that D.N. Smad4-100T is capable of blocking TGF-beta1 action in the regulation of thyroid-specific genes expression. Such action is possible by blocking nuclear translocation of Smad4 and Smad2. |
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Authors:
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Arianna Nicolussi; Sonia D'Inzeo; Maria Santulli; Giulia Colletta; Anna Coppa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 207 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-15 Completed Date: 2004-05-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 1-11 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine and Pathology, University 'La Sapienza', v.le Regina Elena 324, 00161, Rome, Italy |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantigens / genetics, physiology Blotting, Northern Blotting, Western Cell Differentiation / drug effects* Cell Line Clone Cells Cysteine Endopeptidases / genetics, metabolism DNA-Binding Proteins / genetics, metabolism, physiology Down-Regulation Gene Expression Regulation / drug effects Genetic Vectors / genetics Immunohistochemistry Iodide Peroxidase / genetics, physiology Iron-Binding Proteins / genetics, physiology Luciferases / genetics, metabolism Mutation / genetics Nuclear Proteins / genetics, metabolism Paired Box Transcription Factors Phosphorylation Rats Recombinant Fusion Proteins / genetics, metabolism Signal Transduction / physiology Smad2 Protein Smad4 Protein Symporters / genetics, physiology Thyroid Gland / cytology, drug effects* Thyrotropin / pharmacology Trans-Activators / genetics, metabolism, physiology Transcription Factors / genetics, metabolism Transfection Transforming Growth Factor beta / pharmacology*, physiology Transforming Growth Factor beta1 |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/DNA-Binding Proteins; 0/Iron-Binding Proteins; 0/Madh2 protein, rat; 0/Madh4 protein, rat; 0/Nuclear Proteins; 0/Paired Box Transcription Factors; 0/Pax8 protein, rat; 0/Recombinant Fusion Proteins; 0/Smad2 Protein; 0/Smad4 Protein; 0/Symporters; 0/TGFB1 protein, human; 0/Tgfb1 protein, rat; 0/Trans-Activators; 0/Transcription Factors; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/sodium-iodide symporter; 0/thyroid nuclear factor 1; 9002-71-5/Thyrotropin; EC 1.11.1.7/TPO protein, human; EC 1.11.1.8/Iodide Peroxidase; EC 1.13.12.-/Luciferases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.-/cysteine proteinase I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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