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TGBp3 triggers the unfolded protein response and SKP1-dependent programmed cell death.
MedLine Citation:
PMID:  23458484     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The Potato virus X (PVX) triple gene block protein 3 (TGBp3), an 8-kDa membrane binding protein, aids virus movement and induces the unfolded protein response (UPR) during PVX infection. TGBp3 was expressed from the Tobacco mosaic virus (TMV) genome (TMV-p3), and we noted the up-regulation of SKP1 and several endoplasmic reticulum (ER)-resident chaperones, including the ER luminal binding protein (BiP), protein disulphide isomerase (PDI), calreticulin (CRT) and calmodulin (CAM). Local lesions were seen on leaves inoculated with TMV-p3, but not TMV or PVX. Such lesions were the result of TGBp3-elicited programmed cell death (PCD), as shown by an increase in reactive oxygen species, DNA fragmentation and induction of SKP1 expression. UPR-related gene expression occurred within 8 h of TMV-p3 inoculation and declined before the onset of PCD. TGBp3-mediated cell death was suppressed in plants that overexpressed BiP, indicating that UPR induction by TGBp3 is a pro-survival mechanism. Anti-apoptotic genes Bcl-xl, CED-9 and Op-IAP were expressed in transgenic plants and suppressed N gene-mediated resistance to TMV, but failed to alleviate TGBp3-induced PCD. However, TGBp3-mediated cell death was reduced in SKP1-silenced Nicotiana benthamiana plants. The combined data suggest that TGBp3 triggers the UPR and elicits PCD in plants.
Authors:
Chang-Ming Ye; Shaorong Chen; Mark Payton; Martin B Dickman; Jeanmarie Verchot
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular plant pathology     Volume:  14     ISSN:  1364-3703     ISO Abbreviation:  Mol. Plant Pathol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954969     Medline TA:  Mol Plant Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  241-55     Citation Subset:  IM    
Copyright Information:
© 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD.
Affiliation:
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
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