Document Detail


TEQ-value determinations of animal feed; emphasis on the CALUX bioassay validation.
MedLine Citation:
PMID:  18969559     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Polyhalogenated aromatic hydrocarbons, such as polychlorinated dibenzo-p-dioxins are a large and diverse group of environmental pollutants. Their tendency to accumulate in the food chain and their toxicity make monitoring necessary. The reference analysis method is laborious and very expensive, therefore cheap and rapid bioassays have been developed. The chemical-activated luciferase bioassay (CALUX) bioassay uses a recombinant cell line, which responds to dioxins and dioxin-like molecules with Ah receptor (AhR)-dependent induction of firefly luciferase in a dose related response. The CALUX was tested for its use in the screening of feed. Aliquots of 20g of enriched feed were extracted with a toluene:methanol mixture (20:4 v/v) and extracts were defatted on 33% H(2)SO(4) silica columns and purified on carbon columns. Only the dioxin and furan fraction was analysed, the PCB fraction was discarded. The precision of the method is acceptable and in compliance with an R.S.D. <30% as suggested for cell-based bioassays in the Commission Directive 2002/70/EC of July 2002. The results evidence good agreement between TEQ-values obtained by either CALUX or GC-HRMS. The method is now routinely in use for a feed screening programme designed by the Federal Agency for the Safety of the Food chain. Approximately, 25 samples are analysed weekly. From the obtained results approximately 10% was confirmed by GC-HRMS. The false positive ratio is 1% and no false negatives were found, making the use of the CALUX technology advantageous.
Authors:
H Vanderperren; N Van Wouwe; S Behets; I Windal; I Van Overmeire; A Fontaine
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  63     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1277-80     Citation Subset:  -    
Affiliation:
Federal Agency for the Safety of the Food Chain, 17 Leuvensesteenweg, B-3080 Tervuren, Belgium.
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