Document Detail

TEMPO EC for the enumeration of Escherichia coli in foods: collaborative study.
MedLine Citation:
PMID:  20480906     Owner:  NLM     Status:  MEDLINE    
The automated method for enumeration of Escherichia coli, TEMPO EC, in foods uses a dehydrated culture medium and enumeration card containing 48 wells across three different dilutions for the automatic determination of the most probable number (MPN). The alternative method was compared in a multilaboratory collaborative study to AOAC Official Method 966.24. Six food types were artificially contaminated with E. coli: raw ground beef, bagged lettuce, cooked chicken, pasteurized crabmeat, frozen green beans, and pasteurized whole milk. All foods were analyzed for E. coli counts by 11 collaborating laboratories throughout the United States. Test portions from the six food types each contaminated at four different contamination levels were evaluated. The study demonstrated that the TEMPO EC method is a reliable, automated assay for the enumeration of E. coli in foods.
Erin Crowley; Patrick Bird; Marianne Torontali; Katherine Goetz; James Agin; David Goins; Ronald Johnson; M Achen; A Barlowe; M Clark; J Colón-Reveles; K Dixon; K Fisher; P Hanson; R Jechorek; L Johnson; M Kelly; S Kim; H Kohler; D Kondratko; B Kupski; K McCallum; J Mills; F Mohnke; B Moon; B Olson; C Reed; J Sauter; L Thompson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  2011-02-14    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  576-86     Citation Subset:  IM    
Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA.
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MeSH Terms
Chickens / microbiology
Colony Count, Microbial / methods*
Escherichia coli / metabolism*
Food Analysis
Food Contamination*
Food Microbiology*
Laboratories / standards
Meat / microbiology
Reproducibility of Results
Research Design
Seafood / microbiology
Vegetables / microbiology
Erratum In:
J AOAC Int. 2010 Nov-Dec;93(6):188A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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