| TEMPO EC for the enumeration of Escherichia coli in foods: collaborative study. | |
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MedLine Citation:
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PMID: 20480906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The automated method for enumeration of Escherichia coli, TEMPO EC, in foods uses a dehydrated culture medium and enumeration card containing 48 wells across three different dilutions for the automatic determination of the most probable number (MPN). The alternative method was compared in a multilaboratory collaborative study to AOAC Official Method 966.24. Six food types were artificially contaminated with E. coli: raw ground beef, bagged lettuce, cooked chicken, pasteurized crabmeat, frozen green beans, and pasteurized whole milk. All foods were analyzed for E. coli counts by 11 collaborating laboratories throughout the United States. Test portions from the six food types each contaminated at four different contamination levels were evaluated. The study demonstrated that the TEMPO EC method is a reliable, automated assay for the enumeration of E. coli in foods. |
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Authors:
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Erin Crowley; Patrick Bird; Marianne Torontali; Katherine Goetz; James Agin; David Goins; Ronald Johnson; M Achen; A Barlowe; M Clark; J Colón-Reveles; K Dixon; K Fisher; P Hanson; R Jechorek; L Johnson; M Kelly; S Kim; H Kohler; D Kondratko; B Kupski; K McCallum; J Mills; F Mohnke; B Moon; B Olson; C Reed; J Sauter; L Thompson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of AOAC International Volume: 93 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2010 Mar-Apr |
Date Detail:
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Created Date: 2010-05-19 Completed Date: 2010-06-14 Revised Date: 2011-02-14 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 576-86 Citation Subset: IM |
Affiliation:
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Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Automation Cattle Chickens / microbiology Colony Count, Microbial / methods* Escherichia coli / metabolism* Food Analysis Food Contamination* Food Microbiology* Laboratories / standards Meat / microbiology Reproducibility of Results Research Design Seafood / microbiology Vegetables / microbiology |
| Comments/Corrections | |
Erratum In:
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J AOAC Int. 2010 Nov-Dec;93(6):188A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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