Document Detail


TEM immunogold staining of C3 from plasma onto titanium oxides.
MedLine Citation:
PMID:  1717473     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunogold staining in conjunction with TEM was used to observe C3 adsorption from plasma in relation to the underlying titanium structure of thermal, anodic, and electropolished oxides. Heat treatments and oxide thickness were found to have no significant effect on the adsorption behavior of C3, while surface oxide type possibly has. Surface concentration of C3 was found to be time- and plasma concentration-dependent. Evidence is given for the possible involvement of C3 in protein exchange, i.e., the Vroman effect. Diluted plasma resulted in a random distribution of gold colloids, whereas clustering occurred with undiluted plasma. Although C3 concentrations present on grain boundaries followed the same trend as that found on the surface, C3 was found to have a higher grain boundary than bulk concentration for 0.1% plasma.
Authors:
M E McAlarney; R Skalak; S Kim; D Neugroschl; E S Machlin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  25     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-11-08     Completed Date:  1991-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  845-64     Citation Subset:  IM    
Affiliation:
Department of Prosthodontics, School of Dental and Oral Surgery, Columbia University, New York, New York 10032.
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MeSH Terms
Descriptor/Qualifier:
Adsorption / drug effects
Biocompatible Materials*
Complement C3c
Gold
Immunohistochemistry
Microscopy, Electron
Staining and Labeling
Time Factors
Titanium*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 13463-67-7/titanium dioxide; 7440-32-6/Titanium; 7440-57-5/Gold; 80295-44-9/Complement C3c

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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