| TCR kappa, a new splicing of the murine TCR zeta gene locus, is modulated by glucocorticoid treatment. | |
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MedLine Citation:
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PMID: 10395068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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T-cell receptor (TCR) is a multichain receptor in which the TCRzeta subunit is important for membrane assembly and signal transduction. Four alternative splicings of the murine TCRzeta gene locus have been previously described. We here describe a new alternative splicing of murine TCRzeta gene, TCRkappa, cloned by RT-PCR, that is encoded by exons 1-7, a portion of exon 9 and the whole exon 10 of TCRzeta gene. The protein encoded by TCRkappa mRNA is identical to that encoded by TCReta mRNA, because the stop codon is present in the exon 9 before splicing with exon 10. RNAse protection assays carried out on total RNA from thymocytes indicate that TCRkappa mRNA is 1 half with respect to TCReta mRNA, suggesting that TCRkappa mRNA contributes to determine the TCReta protein levels. The 3' untranslated region of TCRkappa mRNA is different from that of TCReta and this might lead to different t(1/2) for each species in vivo. We also show that dexamethasone (DEX), a synthetic glucocorticoid hormone, increases the amount of TCRkappa in the hybridoma T-cell line 3DO (about 5-fold increase), as indicated by reverse transcriptase-polymerase chain reaction (RT-PCR) and RNAse protection assays. This newly described effect of DEX may constitute a further molecular mechanism that contributes to its immunomodulating activity. |
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Authors:
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L Giunchi; G Nocentini; S Ronchetti; A Bartoli; C Riccardi; G Migliorati |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 195 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-10-28 Completed Date: 1999-10-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 47-53 Citation Subset: IM |
Affiliation:
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Department of Clinical and Experimental Medicine (DMCS), Perugia University Medical School, Italy. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U56258 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing*
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drug effects Amino Acid Sequence Animals Base Sequence Cloning, Molecular Dexamethasone / pharmacology Glucocorticoids / pharmacology* Membrane Proteins / genetics* Mice Mice, Inbred C3H Molecular Sequence Data RNA, Messenger / biosynthesis, drug effects Receptors, Antigen, T-Cell / biosynthesis, genetics* Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / metabolism Thymus Gland / cytology, drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glucocorticoids; 0/Membrane Proteins; 0/RNA, Messenger; 0/Receptors, Antigen, T-Cell; 0/antigen T cell receptor, eta-kappa; 0/antigen T cell receptor, zeta chain; 50-02-2/Dexamethasone |
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