Document Detail


TATA-binding protein-related factor 2 is localized in the cytoplasm of mammalian cells and much of it migrates to the nucleus in response to genotoxic agents.
MedLine Citation:
PMID:  17085973     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TBP (TATA-binding protein)-related factor 2 (TRF2) regulates transcription during a nuber of cellular processes. We previously demonstrated that it is localized in the cytoplasm and is translocated to the nucleus by DNA-damaging agents. However, the cytoplasmic localization of TRF2 is controversial. In this study, we reconfirmed its cytoplasmic localization in various ways and examined its nuclear migration. Stresses such as heat shock, redox agents, heavy metals, and osmotic shock did not affect localization whereas genotoxins such as methyl methanesulfonate (MMS), cisplatin, etoposide, and hydroxyurea caused it to migrate to the nucleus. Adriamycin, mitomycin C and gamma-rays had no obvious effect. We determined optimal conditions for the nuclear migration. The proportions of cells with nuclei enriched for TRF2 were 25-60% and 5-10% for stressed cells and control cells, respectively. Nuclear translocation was observed after 1 h, 4 h and 12 h for cisplatin, etoposide and MMS and hydroxyurea, respectively. The association of TRF2 with the chromatin and promoter region of the proliferating cell nuclear antigen (PCNA) gene, a putative target of TRF2, was increased by MMS treatment. Thus TRF2 may be involved in genotoxin-induced transcriptional regulation.
Authors:
Kyoung-ae Park; Yuji Tanaka; Yusuke Suenaga; Taka-aki Tamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  22     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-11-06     Completed Date:  2006-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  203-9     Citation Subset:  IM    
Affiliation:
Department of Biology, Faculty of Science, Chiba University, Chiba 263-8522, Japan.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus / drug effects
Animals
COS Cells
Cell Nucleus / metabolism*
Cercopithecus aethiops
Chromatin / chemistry
Cisplatin / pharmacology
Cytoplasm / chemistry*
DNA Damage*
Hela Cells
Histones / analysis
Humans
Methyl Methanesulfonate / pharmacology
Mice
Mutagens / pharmacology*
NIH 3T3 Cells
Proliferating Cell Nuclear Antigen / metabolism
TATA Box Binding Protein-Like Proteins / analysis*,  metabolism*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Chromatin; 0/Histones; 0/Mutagens; 0/Proliferating Cell Nuclear Antigen; 0/TATA Box Binding Protein-Like Proteins; 15663-27-1/Cisplatin; 66-27-3/Methyl Methanesulfonate

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