| TATA-binding protein-related factor 2 is localized in the cytoplasm of mammalian cells and much of it migrates to the nucleus in response to genotoxic agents. | |
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MedLine Citation:
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PMID: 17085973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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TBP (TATA-binding protein)-related factor 2 (TRF2) regulates transcription during a nuber of cellular processes. We previously demonstrated that it is localized in the cytoplasm and is translocated to the nucleus by DNA-damaging agents. However, the cytoplasmic localization of TRF2 is controversial. In this study, we reconfirmed its cytoplasmic localization in various ways and examined its nuclear migration. Stresses such as heat shock, redox agents, heavy metals, and osmotic shock did not affect localization whereas genotoxins such as methyl methanesulfonate (MMS), cisplatin, etoposide, and hydroxyurea caused it to migrate to the nucleus. Adriamycin, mitomycin C and gamma-rays had no obvious effect. We determined optimal conditions for the nuclear migration. The proportions of cells with nuclei enriched for TRF2 were 25-60% and 5-10% for stressed cells and control cells, respectively. Nuclear translocation was observed after 1 h, 4 h and 12 h for cisplatin, etoposide and MMS and hydroxyurea, respectively. The association of TRF2 with the chromatin and promoter region of the proliferating cell nuclear antigen (PCNA) gene, a putative target of TRF2, was increased by MMS treatment. Thus TRF2 may be involved in genotoxin-induced transcriptional regulation. |
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Authors:
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Kyoung-ae Park; Yuji Tanaka; Yusuke Suenaga; Taka-aki Tamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecules and cells Volume: 22 ISSN: 1016-8478 ISO Abbreviation: Mol. Cells Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-11-06 Completed Date: 2006-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9610936 Medline TA: Mol Cells Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 203-9 Citation Subset: IM |
Affiliation:
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Department of Biology, Faculty of Science, Chiba University, Chiba 263-8522, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus
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drug effects Animals COS Cells Cell Nucleus / metabolism* Cercopithecus aethiops Chromatin / chemistry Cisplatin / pharmacology Cytoplasm / chemistry* DNA Damage* Hela Cells Histones / analysis Humans Methyl Methanesulfonate / pharmacology Mice Mutagens / pharmacology* NIH 3T3 Cells Proliferating Cell Nuclear Antigen / metabolism TATA Box Binding Protein-Like Proteins / analysis*, metabolism* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Histones; 0/Mutagens; 0/Proliferating Cell Nuclear Antigen; 0/TATA Box Binding Protein-Like Proteins; 15663-27-1/Cisplatin; 66-27-3/Methyl Methanesulfonate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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