| TAT-peptide modified liposomes: preparation, characterization, and cellular interaction. | |
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MedLine Citation:
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PMID: 20072893 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In general, cellular internalization of macromolecular drugs encapsulated in liposomes proceeds via endocytosis. This potentially leads to degradation of the liposome-encapsulated macromolecular content within the endosomal/lysosomal compartment. Therefore, bypassing the endocytic route by conferring a direct plasma membrane translocation property to the liposomes would be very beneficial. Cell penetrating peptides, e.g. TAT-peptide, are exploited in the drug delivery field for their capacity of plasma membrane translocation. Here, we describe the preparation of TAT-peptide modified liposomes and their cellular interaction using live cell flow cytometry and imaging techniques. |
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Authors:
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Marjan M Fretz; Gert Storm |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 605 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 349-59 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Endocytosis Humans Liposomes / chemistry*, pharmacokinetics* tat Gene Products, Human Immunodeficiency Virus / chemistry*, pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 0/tat Gene Products, Human Immunodeficiency Virus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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