Document Detail


TAF4b and Jun/activating protein-1 collaborate to regulate the expression of integrin alpha6 and cancer cell migration properties.
MedLine Citation:
PMID:  20353996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The TAF4b subunit of the transcription factor IID, which has a central role in transcription by polymerase II, is involved in promoter recognition by selective recruitment of activators. The activating protein-1 (AP-1) family members participate in oncogenic transformation via gene regulation. Utilizing immunoprecipitation of endogenous protein complexes, we documented specific interactions between Jun family members and TATA box binding protein-associated factors (TAF) in colon HT29 adenocarcinoma cells. Particularly, TAF4b and c-Jun were found to colocalize and interact in the nucleus of advanced carcinoma cells and in cells with epithelial-to-mesenchymal transition (EMT) characteristics. TAF4b was found to specifically regulate the AP-1 target gene involved in EMT integrin alpha6, thus altering related cellular properties such as migration potential. Using a chromatin immunoprecipitation approach in colon adenocarcinoma cell lines, we further identified a synergistic role for TAF4b and c-Jun and other AP-1 family members on the promoter of integrin alpha6, underlining the existence of a specific mechanism related to gene expression control. We show evidence for the first time of an interdependence of TAF4b and AP-1 family members in cell type-specific promoter recognition and initiation of transcription in the context of cancer progression and EMT.
Authors:
Margarita Kalogeropoulou; Angeliki Voulgari; Vassiliki Kostourou; Raphael Sandaltzopoulos; Rivka Dikstein; Irwin Davidson; Laszlo Tora; Alexander Pintzas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-30
Journal Detail:
Title:  Molecular cancer research : MCR     Volume:  8     ISSN:  1557-3125     ISO Abbreviation:  Mol. Cancer Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-16     Completed Date:  2010-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101150042     Medline TA:  Mol Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  554-68     Citation Subset:  IM    
Copyright Information:
(c) 2010 AACR.
Affiliation:
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Cell Dedifferentiation / genetics
Cell Line, Tumor
Cell Movement / genetics
Cell Nucleus / genetics,  metabolism
Cell Transformation, Neoplastic / genetics,  metabolism
Gene Expression Regulation, Neoplastic / genetics
Humans
Integrin alpha6 / genetics*,  metabolism
Neoplasm Invasiveness / genetics*
Neoplasms / genetics*,  metabolism
Promoter Regions, Genetic / genetics
TATA-Binding Protein Associated Factors / genetics*,  metabolism
Transcription Factor AP-1 / genetics*,  metabolism
Transcription Factor TFIID / genetics*,  metabolism
Transcriptional Activation / genetics*
Chemical
Reg. No./Substance:
0/Integrin alpha6; 0/TAF4B protein, human; 0/TATA-Binding Protein Associated Factors; 0/Transcription Factor AP-1; 0/Transcription Factor TFIID

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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