| T98G glioma cells have nicks in DNA in quiescent phase. | |
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MedLine Citation:
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PMID: 2209724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human glioblastoma-derived cell line, T98G, is arrested in the G1 phase of the cell cycle when serum is deprived. Using this cell line, we investigated the relation between the cell cycle and DNA single-stranded breaks, "nicks," by an in situ nick-translation method. When T98G cells were cultured without serum for 60 h, many small cells with condensed chromatin and scanty cytoplasm appeared. These small cells that were immunohistochemically considered to be in the G0 or early G1 phase had many nicks in DNA. When serum was added, these small cells with nicks disappeared within 1 to 4 h. VP-16, a DNA topoisomerase II inhibitor, delayed the disappearance of these small cells with nicks. This indicated that the action of DNA topoisomerase II on the chromatin is required to repair nicks in T98G glioma cells and to promote the progression from the quiescent to the proliferating phase. |
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Authors:
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T Taki; N Arita; T Hayakawa; T Ohnishi; S Izumoto; H Yamamoto; H Mogami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 190 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1990-11-05 Completed Date: 1990-11-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 212-7 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Osaka University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Surface
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immunology Cell Cycle Cell Line DNA Damage / genetics* DNA Polymerase II / immunology DNA Repair DNA, Neoplasm / analysis, genetics* Flow Cytometry Glioma / chemistry, genetics*, immunology, pathology Humans Immunohistochemistry / methods Interphase Ki-67 Antigen |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/DNA, Neoplasm; 0/Ki-67 Antigen; EC 2.7.7.-/DNA Polymerase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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