Document Detail


T6 positive cells in the peripheral blood of burn patients: are they Langerhans cells precursors?
MedLine Citation:
PMID:  3257514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peripheral blood mononuclear cells of 14 patients suffering thermal injury were separated by affinity chromatography on peanut agglutinin (PNA) coupled to Sepharose macrobeads. The resulting PNA positive subset was 14% of the total mononuclear population. About 30% of these cells were found to coexpress T6(CD1), Ia-like and the myeloid differentiation antigens My4(CDw14) and Mo1(CD11). In comparison, the PNA+ subset from normal blood donors (about 5% of total mononuclear cells) contained mature monocytes that were found to be T6 negative. Electron microscopic studies using immunogold labeling showed that the T6 positive cells were slightly smaller than monocytes but larger than the classical lymphocytes and had common morphologic features with the Langerhans cells of the skin. Considering that patients suffering extensive damage of the epidermis require fast renewal of all skin elements, it is possible that the cells we identified in their peripheral blood are the precursors of the Langerhans cells of the skin en route from bone marrow to the epidermis.
Authors:
Y Gothelf; D Hanau; H Tsur; N Sharon; E Sahar; J P Cazenave; E Gazit
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  90     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-15     Completed Date:  1988-03-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  142-8     Citation Subset:  IM    
Affiliation:
Tissue Typing Laboratory Chaim Sheba Medical Center, Tel Hashomer, Israel.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Monoclonal / immunology
Antigens, Differentiation, T-Lymphocyte / analysis*
Burns / blood*
DNA / analysis
Female
Humans
Langerhans Cells / immunology*
Leukocytes, Mononuclear / immunology*,  ultrastructure
Male
Middle Aged
Stem Cells / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, T-Lymphocyte; 9007-49-2/DNA

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