| T([20]) repeat in the 3'-untranslated region of the MT1X gene: a marker with high sensitivity and specificity to detect microsatellite instability in colorectal cancer. | |
| | |
MedLine Citation:
|
PMID: 22108904 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: Stratifying patients defective in mismatch repair (dMMR) with high microsatellite instability (MSI-H) in colorectal cancer (CRC) is of increasing relevance and may provide a more tailored approach to CRC adjuvant therapy. Here, we describe the discovery of a new MSI marker for colorectal cancer located in the 3'-untranslated region (3'UTR, T20 mononucleotide repeat) of the metallothionein 1X gene (MT1XT20). METHODS: We studied 340 consecutive CRCs using three multiplexed polymerase chain reactions amplifying BAT25, BAT26, TGFBR2, MybT22, BAT40, MT1XT20, NR21, NR24, CAT25, D2S123, D5S346, D17S250, D18S58, CSF1PO, D7S820, and D18S51. Fragments length was evaluated by automated capillary electrophoresis. RESULTS: Based on the NCI/ICG-HNPCC criteria for MSI classification, 40 CRCs were found to be MSI-high (11.8%), 46 (13.5%) CRCs were MSI-low, and 254 CRCs (74.7%) were stable (MSS). MT1XT20 showed very high sensitivity (97.3%) comparable to BAT26 (97.5%) and CAT25 (97.1%) and the best specificity (100%) as well as MybT22 and CAT25. Indeed, MT1XT20 instability was detected in 36 out of 37 cases (97.3%) of MSI-high colorectal cancers, whereas no MT1XT20 alterations were observed in 254 MSS or in 46 MSI-low cases. On the contrary, BAT40 was found to be unstable in 8/46 MSI-low cases, BAT25 in 6/46, BAT26 4/46, NR21 1/46, and NR24 in 1/45. CONCLUSIONS: Our results suggest that MT1XT20 represents a sensitive and specific marker for MSI testing and could be included in a complete set of MSI markers for the confident identification of familial or sporadic dMMR patients in CRCs. |
| | |
Authors:
|
Luca Morandi; Dario de Biase; Michela Visani; Adriana Monzoni; Annalisa Tosi; Mauro Brulatti; Daniela Turchetti; Paola Baccarini; Giovanni Tallini; Annalisa Pession |
Related Documents
:
|
22016704 - Cancer incidence of 2,4-d production workers. 22186794 - The oncolytic poxvirus jx-594 selectively replicates in and destroys cancer cells drive... 22068034 - Targeting regulatory t cells in cancer. 22087614 - A pilot study of website information regarding aromatase inhibitors: dietary supplement... 14719614 - Temporal and gender-related trends in brain metastases from lung and breast cancer. 21443504 - Astemizole: an old anti-histamine as a new promising anti-cancer drug. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-23 |
Journal Detail:
|
Title: International journal of colorectal disease Volume: - ISSN: 1432-1262 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8607899 Medline TA: Int J Colorectal Dis Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Haematology and Oncological Sciences L. and A. Seragnoli, Section of Anatomic Pathology at Bellaria Hospital, University of Bologna, via Altura 3, 40139, Bologna, Italy, l.morandi@ausl.bo.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The haplotypes of TNFRSF17 polymorphisms are associated with colon cancer in a Korean population.
Next Document: DNA promoter methylation status and protein expression of interleukin-8 in human colorectal adenocar...