| Treg cell numbers and function in patients with antibiotic-refractory or antibiotic-responsive Lyme arthritis. | |
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MedLine Citation:
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PMID: 20506317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: In a murine model of antibiotic-refractory Lyme arthritis, the numbers of Treg cells are dramatically reduced. The aim of this study was to examine Treg cell numbers and function in patients with antibiotic-refractory Lyme arthritis. METHODS: CD4+ T cell subsets were enumerated in the peripheral blood (PB) and synovial fluid (SF) of 12 patients with antibiotic-refractory arthritis and 6 patients with antibiotic-responsive arthritis. Treg cell function was examined using Borrelia-specific and nonspecific Treg cell proliferation assays. RESULTS: In both patient groups, interferon-gamma-positive Th1 cells in SF were abundant and enriched (approximately 50% of CD4+ T cells). In patients with antibiotic-refractory arthritis, the median percentages of FoxP3-positive Treg cells were significantly higher in SF than in PB (12% versus 6%; P = 0.03) or in SF from patients with antibiotic-responsive arthritis (12% versus 5%; P = 0.04). Moreover, in the antibiotic-refractory group, a higher percentage of Treg cells in SF correlated with a shorter duration until resolution of arthritis (r = -0.74, P = 0.006). In contrast, patients with fewer Treg cells had suboptimal responses to disease-modifying antirheumatic drugs and a longer duration of arthritis after antibiotic treatment, and they often required synovectomies for arthritis resolution. In each group, Treg cells in SF dampened Borrelia burgdorferi-specific proliferative responses, and in 2 patients with antibiotic-refractory arthritis, Treg cells were functional in nonspecific suppression assays. CONCLUSION: Treg cells were functional in patients with antibiotic-refractory arthritis, and in some patients, higher numbers of these cells in SF appeared to participate in arthritis resolution. However, as in the murine model, patients with antibiotic-refractory arthritis and lower numbers of Treg cells seemed unable to achieve resolution of synovial inflammation. |
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Authors:
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Shiqian Shen; Junghee J Shin; Klemen Strle; Gail McHugh; Xin Li; Lisa J Glickstein; Elise E Drouin; Allen C Steere |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 62 ISSN: 1529-0131 ISO Abbreviation: Arthritis Rheum. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-07-29 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: United States |
Other Details:
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Languages: eng Pagination: 2127-37 Citation Subset: AIM; IM |
Affiliation:
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Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts 02114, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Anti-Bacterial Agents / pharmacology, therapeutic use* Arthritis, Infectious / drug therapy, immunology, pathology* Borrelia burgdorferi / drug effects CD4 Lymphocyte Count Cell Proliferation Child Drug Resistance, Bacterial* / drug effects Female Humans Lyme Disease / drug therapy, immunology, pathology* Lymphocyte Activation Male Middle Aged Synovial Fluid / cytology, immunology T-Lymphocyte Subsets / immunology, pathology T-Lymphocytes, Regulatory / immunology, pathology* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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AR-007258/AR/NIAMS NIH HHS; AR-20358/AR/NIAMS NIH HHS; R01 AR020358-36/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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