Document Detail


T lymphocytes from patients with systemic lupus erythematosus are resistant to induction of autophagy.
MedLine Citation:
PMID:  22835828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autophagy, the cytoprotection mechanism that takes place under metabolic impairment, has been implicated in the pathogenesis of autoimmunity. Here, we investigated the spontaneous and induced autophagic behavior of T lymphocytes from patients with systemic lupus erythematosus (SLE) compared with that of T lymphocytes from healthy donors by measuring the autophagy marker microtubule-associated protein 1 light chain 3 (LC3)-II. No significant differences in spontaneous autophagy were found between T lymphocytes from patients with SLE and from healthy donors, apart from CD4(+) naive T cells from patients with SLE in which constitutively higher levels of autophagy (P<0.001) were detected. At variance, whereas treatment of T lymphocytes from healthy donors with serum IgG from patients with SLE resulted in a 2-fold increase in LC3-II levels (P<0.001), T lymphocytes from SLE patients were resistant to autophagic induction and also displayed an up-regulation of genes negatively regulating autophagy, e.g., α-synuclein. These findings could open new perspectives in the search for pathogenetic determinants of SLE progression and in the development of therapeutic strategies aimed to recover T-cell compartment homeostasis by restoring autophagic susceptibility.
Authors:
Cristiano Alessandri; Cristiana Barbati; Davide Vacirca; Paola Piscopo; Annamaria Confaloni; Massimo Sanchez; Angela Maselli; Tania Colasanti; Fabrizio Conti; Simona Truglia; Andras Perl; Guido Valesini; Walter Malorni; Elena Ortona; Marina Pierdominici
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-26
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  26     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-02     Completed Date:  2013-01-29     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4722-32     Citation Subset:  IM    
Affiliation:
Lupus Clinic, Dipartimento di Medicina Interna e Specialità Mediche, Sapienza University, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Autoantibodies
Autophagy / physiology*
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation
Humans
Immunoglobulin G / blood
Lupus Erythematosus, Systemic / immunology*
Male
Middle Aged
Real-Time Polymerase Chain Reaction
T-Lymphocytes / physiology*
Young Adult
Grant Support
ID/Acronym/Agency:
AI048079/AI/NIAID NIH HHS; AI072648/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunoglobulin G
Comments/Corrections

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