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T-lymphocyte-induced, fas-mediated apoptosis is associated with early keratinocyte differentiation.
MedLine Citation:
PMID:  19645855     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The development of eczematous lesions is thought to be due in part to a breakdown in skin barrier function as a result of T lymphocytes (T cells) invading the skin causing epidermal keratinocyte apoptosis. In this study, we investigated the interaction of T cells and keratinocytes on apoptosis and terminal differentiation using an in vitro co-culture system. Experiments were performed using the HaCaT keratinocyte cell line or normal human epidermal keratinocytes. Activated human peripheral blood-derived T cells were found to induce Fas-dependent keratinocyte apoptosis by up to sixfold. Increased Fas was associated with increased IFN-gamma. The T-cell apoptotic signal was found to target preferentially keratinocytes in the very early stages of terminal differentiation, such as those with low levels of alpha 6-integrin expression, and result in subsequent increased caspase 3 activity. This observation was accompanied by a marked increase in keratinocyte ICAM-1 expression and its ligand LFA-1 on T cells. Our data suggest that T cells may initiate the onset of keratinocyte terminal differentiation making them more susceptible to Fas-dependent cell death signals delivered by the T cells.
Authors:
Ilse S Daehn; Antiopi Varelias; Timothy E Rayner
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Publication Detail:
Type:  Journal Article     Date:  2009-07-23
Journal Detail:
Title:  Experimental dermatology     Volume:  19     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  372-80     Citation Subset:  IM    
Affiliation:
Women's & Children's Health Research Institute, Women's and Children's Hospital, North Adelaide, SA, Australia. ilse.daehn@cancer.org.uk
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