Document Detail


T-lymphocyte colony formation by lymphocytes from patients with aplastic anaemia.
MedLine Citation:
PMID:  3494909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T-lymphocyte colonies were cultured using lymphocytes from patients with aplastic anaemia and normal donors to assess their respective proliferative activities. Colony numbers from aplastic patient's cells were lower than from normal donors', though this was not significant. When lymphocytes from patients were co-cultured with normal lymphocytes, inhibition of T-colony formation was observed in 8 out of 12 experiments. As the degree of inhibition was greater than if patient cells grew no colonies, then, clearly, normal T-colony formation was inhibited. This ability of patients' lymphocytes to suppress lymphopoiesis might account for the low levels of patient T-colony formation, as well as low in vivo numbers of lymphocytes found in patients with aplastic anaemia. The role of patients' lymphocytes in causing marrow aplasia was investigated. Although the incorporation of patients' lymphocytes in normal granulocyte-macrophage (GM) colony-forming systems inhibited colony growth, in only 1 out of 8 patients was this inhibition significantly greater than that caused by the addition of normal lymphocytes to GM colony systems. Therefore, lymphocytes may not be the primary cause of aplastic anaemia, except for a few rare cases.
Authors:
K T Ennis; T C Morris; A E Irvine; H D Alexander
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Natural immunity and cell growth regulation     Volume:  6     ISSN:  0254-7600     ISO Abbreviation:  Nat. Immun. Cell Growth Regul.     Publication Date:  1987  
Date Detail:
Created Date:  1987-05-26     Completed Date:  1987-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8407979     Medline TA:  Nat Immun Cell Growth Regul     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  45-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Anemia, Aplastic / blood*
Cell Communication
Colony-Forming Units Assay*
Female
Granulocytes / cytology
Humans
Lymphocytes / cytology*
Macrophages / cytology
Male
Middle Aged
T-Lymphocytes / classification,  cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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