| T-jump fluorescence relaxation study of the binding of ethidium cation to natural DNA. | |
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MedLine Citation:
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PMID: 8240376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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T-jump chemical relaxation experiments with fluorescence detection were carried out on the binding of ethidium cation to calf thymus DNA over the temperature range from 11 to 35 degrees C for ethidium concentrations from 500 nM to 2 microM, DNA concentrations from 7.5 to 60 microM (base pairs), and KCl concentrations from 20 to 500 mM. In contrast to the findings of earlier investigations, no salt or DNA concentration or temperature dependences were seen that would support a mechanism of intercalation directly from solution or from an electrostatically bound exterior site in rapid equilibrium with solution. It is suggested that the rate-determining process of intercalation in vivo involves large scale dynamics of the DNA itself. |
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Authors:
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R R Monaco; W C Gardiner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 196 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-12-09 Completed Date: 1993-12-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 975-83 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, University of Texas at Austin 78712. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle DNA / chemistry* Ethidium / chemistry* Kinetics Osmolar Concentration Spectrometry, Fluorescence Thermodynamics |
| Chemical | |
Reg. No./Substance:
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3546-21-2/Ethidium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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