Document Detail


T cells in psoriatic lesional skin that survive conventional therapy with NB-UVB radiation display reduced IFN-gamma expression.
MedLine Citation:
PMID:  15024577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The type 1 T cell-derived cytokine interferon gamma (IFN-gamma) is overexpressed in psoriatic lesional skin. Recently, we have shown that a single high erythemal dose of broad-band ultraviolet B (UVB) irradiation reduces type 1 and favors type 2, i.e. interleukin-4 (IL-4), cytokine expression in normal and psoriatic skin. In this study, we wanted to see whether conventional narrow-band UVB (NB-UVB) therapy (i.e. repeated exposure to nonerythemal doses) also affects type 1/type 2 cytokine expression of T cells present in chronic plaque type psoriatic lesions. Staining of cryostat sections showed decreased expression of both IFN-gamma and IL-4 in situ after NB-UVB therapy. CD4(+) dermal T cell lines, derived from psoriatic lesional skin, displayed significantly decreased intracellular IFN-gamma expression during and after NB-UVB therapy as compared to pretreatment values. Intracellular IL-4 expression was increased in most patients after therapy. Analysis of the supernatants of these stimulated dermal T cells revealed that IFN-gamma production decreased significantly following NB-UVB therapy, whereas IL-4 expression increased in the T cell supernatants from most patients, confirming the intracellular determinations. In addition, IL-10 and transforming growth factor-beta levels in the supernatants appeared to be increased in the majority of patients following UVB therapy. Apart from the well-known killing effect of UVB on T cells, our results show that the improvement in psoriatic skin following NB-UVB therapy is also due to a reduced capacity of the surviving dermal T cells to express the proinflammatory cytokine IFN-gamma.
Authors:
Gamze Piskin; Regien M R Sylva-Steenland; Jan D Bos; Marcel B M Teunissen
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Publication Detail:
Type:  Journal Article     Date:  2004-03-16
Journal Detail:
Title:  Archives of dermatological research     Volume:  295     ISSN:  0340-3696     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-12     Completed Date:  2004-12-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  509-16     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Academic Medical Center, University of Amsterdam, Room L3-365, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. g.piskin@amc.uva.nl
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MeSH Terms
Descriptor/Qualifier:
Humans
Interferon-gamma / biosynthesis*
Interleukin-4 / biosynthesis
Psoriasis / immunology,  radiotherapy*
Skin / immunology,  radiation effects
T-Lymphocytes / immunology*
Ultraviolet Therapy*
Chemical
Reg. No./Substance:
207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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