Document Detail


T cells in G1 provide a memory-like response to secondary stimulation.
MedLine Citation:
PMID:  15778358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The commitment of naive T cells to proliferate is a function of the strength and duration of stimuli mediated by the TCR and coreceptors. Ranges of 2-20 h of stimulation have been reported as necessary in vitro. Whether T cells actually experience uninterrupted stimulation for such long periods under physiological conditions is controversial. Here we ask whether commitment to proliferate requires continuous stimulation, or can T cells integrate intermittent periods of stimulation. T cells were stimulated for two short-term (subthreshold) periods (5-7 h) either sequentially or separated by an interval of rest. Naive lymph node T cells were able to integrate interrupted stimulation, even when the duration of rest was as long as 2 days. Furthermore, when short-term-stimulated T cells were separated by density, three populations were observed: low density blasts, intermediate density G(1) cells, and high density G(0) cells. Low density cells progressed to division without further stimulation, whereas G(0) and G(1) cells remained undivided. However, after a period of rest, a second subthreshold stimulation caused the G(1) but not the G(0) fraction to quickly proceed through the cell cycle. We conclude that noncycling T cells in the G(1) phase of the cell cycle remain in a state of readiness for prolonged periods of time, and may represent a population of memory-like effectors capable of responding rapidly to antigenic challenge.
Authors:
Ivana Munitic; Philip E Ryan; Jonathan D Ashwell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  174     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-21     Completed Date:  2005-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4010-8     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis
Cell Cycle / immunology
Cell Proliferation
G0 Phase / immunology
G1 Phase / immunology*
Immunologic Memory*
Interleukin-2 / biosynthesis
Lymphocyte Activation / immunology
Mice
Mice, Inbred C57BL
T-Lymphocytes / cytology,  immunology*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Interleukin-2

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