Document Detail


T cell-specific negative regulation of transcription of the human cytokine IL-4.
MedLine Citation:
PMID:  1541828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IL-4 secreted by activated T cells is a pleiotropic cytokine affecting growth and differentiation of diverse cell types such as T cells, B cells, and mast cells. We investigated the upstream regulatory elements of the human IL-4 promoter. A novel T cell-specific negative regulatory element (NRE) composed of two protein-binding sites were mapped in the 5' flanking region of the IL-4 gene: -311CTCCCTTCT-303 (NRE-I) and -288CTTTTTGCTT-TGC-300 (NRE-II). A T cell-specific protein Neg-1 and a ubiquitous protein Neg-2 binding to NRE-I and NRE-II, respectively, were identified. Furthermore, a positive regulatory element was found 45 bp downstream of the NRE. The enhancer activity of the PRE was completely suppressed when the NRE was present. These data suggest that IL-4 promoter activity is normally down-regulated by an NRE via repression of the enhancer positive regulatory element. These data may have implications for the stringent control of IL-4 expression in T cells.
Authors:
M Li-Weber; A Eder; H Krafft-Czepa; P H Krammer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  148     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-04-09     Completed Date:  1992-04-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1913-8     Citation Subset:  AIM; IM    
Affiliation:
Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Mutational Analysis
DNA-Binding Proteins / metabolism
Gene Expression Regulation
Humans
Interleukin-4 / genetics*
Mice
Molecular Sequence Data
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Repressor Proteins / metabolism
T-Lymphocytes / physiology*
Transcription Factors / metabolism
Transcription, Genetic
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Repressor Proteins; 0/Transcription Factors; 207137-56-2/Interleukin-4

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