| T cell selective apoptosis by a novel immunosuppressant, FTY720, is closely regulated with Bcl-2. | |
| | |
MedLine Citation:
|
PMID: 12429567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
1. A novel immunosuppressant FTY720 caused a significant decrease in peripheral T lymphocytes, but not in B lymphocytes upon oral administration. This decrease was mainly a result of FTY720-induced apoptosis. In this study, we confirmed FTY720-induced T cell selective apoptosis using lymphoma cell lines in vitro. 2. Viability loss, DNA fragmentation, Annexin V binding, and caspases activation (caspase-3, -8, and -9) were observed in Jurkat cells (T lymphoma cells), but not significantly in BALL-1 cells (B lymphoma cells). These results indicated that FTY720 selectively induced apoptosis in T cell lymphoma to a greater extent than in B cell lymphoma, a finding that is similar to the result observed when FTY720 was treated with T lymphocytes and B lymphocytes in vitro. 3. FTY720 released cytochrome c from mitochondria in Jurkat intact cells as well as from isolated Jurkat mitochondria directly, but not from mitochondria in BALL-1 cells nor from isolated BALL-1 mitochondria. 4. BALL-1 cells and B cells had more abundant mitochondria-localized anti-apoptotic protein Bcl-2 than did Jurkat cells and T cells. 5. FTY720-induced apoptosis is inhibited by the overexpression of Bcl-2, suggesting that the cellular Bcl-2 level regulates the sensitivity to FTY720. |
| | |
Authors:
|
Yukitoshi Nagahara; Masahiko Ikekita; Takahisa Shinomiya |
Related Documents
:
|
11848517 - Topotecan (hycamtin) responsiveness in human renal carcinoma cell lines of the clear ce... 15967637 - Constitutive activation of stat proteins in the hdlm-2 and l540 hodgkin lymphoma-derive... 17182797 - Activation of caspase-8 contributes to 3,3'-diindolylmethane-induced apoptosis in colon... 22618587 - Intrastriatal b-cell administration limits infarct size after stroke in b-cell deficien... 6150577 - Electron microscopic observations on primary hepatocyte cultures infected with herpes s... 16886087 - Intracellular photodynamic therapy with photosensitizer-nanoparticle conjugates: cancer... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: British journal of pharmacology Volume: 137 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2002 Dec |
Date Detail:
|
Created Date: 2002-11-13 Completed Date: 2003-05-20 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
|
Languages: eng Pagination: 953-62 Citation Subset: IM |
Affiliation:
|
Division of Radio Isotopes and Biosafety Research, National Research Institute for Child Health and Development, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8567, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects* B-Lymphocytes / cytology, drug effects Cell Survival / drug effects Cytochrome c Group / secretion Dose-Response Relationship, Drug Humans Immunosuppressive Agents / pharmacology* Jurkat Cells / drug effects Mitochondria / drug effects, metabolism Propylene Glycols / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics, physiology* Rats Sphingosine / analogs & derivatives T-Lymphocytes / cytology, drug effects* Transfection Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Cytochrome c Group; 0/Immunosuppressive Agents; 0/Propylene Glycols; 0/Proto-Oncogene Proteins c-bcl-2; 123-78-4/Sphingosine; 162359-55-9/fingolimod |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inhibitory effect of diazepam on muscarinic receptor-stimulated inositol 1,4,5-trisphosphate product...
Next Document: Functional consequences of perinatal exposure to 3,4-methylenedioxymethamphetamine in rat brain.