Document Detail

T cell rescue of monocytes from apoptosis: role of the CD40-CD40L interaction and requirement for CD40-mediated induction of protein tyrosine kinase activity.
MedLine Citation:
PMID:  8929557     Owner:  NLM     Status:  MEDLINE    
Circulating monocytes have a limited life span and will undergo apoptosis in the absence of specific stimuli. Recent studies have demonstrated that monocytes can be rescued from apoptosis via lipopolysaccharide (LPS) activation or stimulation with interleukin-1 or tumor necrosis factor-alpha. Based on previous studies from our laboratory, we hypothesized that, in nonseptic (e.g., autoimmune) inflammation, the presence of activated T cells may enhance monocyte longevity through T cell contact-dependent signaling. Plasma membranes prepared from 6 h activated (TmA) and resting (TmR) purified CD4+ T cells were added to resting elutriation-purified monocytes cultured in serum-free medium. Cells were assayed for degree of apoptosis occurring over a 72-h incubation using both agarose gel electrophoresis and flow cytometry. The addition of TmA (but not TmR) was capable of blocking monocyte apoptosis and the ability of TmA to rescue monocytes was abrogated by the addition of anti-CD40L antibodies. Rescue of monocytes from apoptosis could also be mediated by direct cross-linking of monocyte CD40. Inhibitors of tyrosine kinase activity blocked both TmA and anti-CD40-mediated rescue of monocytes from apoptosis, suggesting a primary role of a tyrosine kinase signaling pathway in the events controlling monocyte longevity.
J Suttles; M Evans; R W Miller; J C Poe; R D Stout; L M Wahl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  60     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-23     Completed Date:  1996-12-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  651-7     Citation Subset:  IM    
Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614, USA.
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MeSH Terms
Antigens, CD40 / physiology*
Apoptosis / physiology*
CD4-Positive T-Lymphocytes / chemistry,  physiology*
CD40 Ligand
Cell Membrane / chemistry*
Cell Separation
Culture Media, Serum-Free
Enzyme Activation
Lymphocyte Activation
Membrane Glycoproteins / physiology*
Monocytes / cytology*
Protein Processing, Post-Translational*
Protein-Tyrosine Kinases / physiology*
Signal Transduction / drug effects,  physiology
Grant Support
Reg. No./Substance:
0/Antigens, CD40; 0/Culture Media, Serum-Free; 0/Membrane Glycoproteins; 147205-72-9/CD40 Ligand; EC Kinases

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