| T cell receptor-induced activation and apoptosis in cycling human T cells occur throughout the cell cycle. | |
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MedLine Citation:
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PMID: 10588669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have found conflicting associations between susceptibility to activation-induced cell death and the cell cycle in T cells. However, most of the studies used potentially toxic pharmacological agents for cell cycle synchronization. A panel of human melanoma tumor-reactive T cell lines, a CD8+ HER-2/neu-reactive T cell clone, and the leukemic T cell line Jurkat were separated by centrifugal elutriation. Fractions enriched for the G0-G1, S, and G2-M phases of the cell cycle were assayed for T cell receptor-mediated activation as measured by intracellular Ca(2+) flux, cytolytic recognition of tumor targets, and induction of Fas ligand mRNA. Susceptibility to apoptosis induced by recombinant Fas ligand and activation-induced cell death were also studied. None of the parameters studied was specific to a certain phase of the cell cycle, leading us to conclude that in nontransformed human T cells, both activation and apoptosis through T cell receptor activation can occur in all phases of the cell cycle. |
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Authors:
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M Karas; T Z Zaks; J L Liu; D LeRoith |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular biology of the cell Volume: 10 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-14 Completed Date: 2000-02-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4441-50 Citation Subset: IM |
Affiliation:
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Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-1770, USA. karas@box-k.nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Antigens, CD3 / immunology, metabolism Antigens, Surface / immunology, metabolism Apoptosis / physiology* Calcium / metabolism Cell Cycle / physiology Fas Ligand Protein Humans Lymphocyte Activation / physiology* Membrane Glycoproteins / immunology, metabolism Receptors, Antigen, T-Cell / metabolism* Recombinant Proteins / metabolism T-Lymphocytes / metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Antigens, Surface; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 0/Receptors, Antigen, T-Cell; 0/Recombinant Proteins; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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