| T cell receptor VB repertoire diversity in patients with immune thrombocytopenia following splenectomy. | |
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MedLine Citation:
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PMID: 12930375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In recent years, a pathophysiological role for T cells in immune thrombocytopenia (ITP) has been established. We applied cDNA size distribution analysis of the T cell receptor (TCR) beta-variable (VB) complementarity-determining region 3 (CDR3) in order to investigate T cell repertoire diversity among immune thrombocytopenia patients who had either responded or not responded to splenectomy, and compared them to normal controls. ITP patients who had had a durable platelet response to splenectomy showed a mean 2.8 +/- 2.1 abnormal CDR3 size patterns per patient, similar to healthy volunteers (2.9 +/- 2.0 abnormal CDR3 size patterns). In contrast, patients unresponsive to splenectomy demonstrated evidence of significantly more clonal T cell expansions than patients who had responded to splenectomy or controls (11.3 +/- 3.3 abnormal CDR3 size patterns per patient; P < 0.001). Of the VB subfamilies analysed, VB3 and VB15 correlated with response or non-response to splenectomy, each demonstrating oligoclonality in non-responding patients (P < 0.05). These findings suggest that removal of the spleen may lead directly or indirectly to reductions in T cell clonal expansions in responders, or that the extent of T cell clonality impacts responsiveness to splenectomy in patients with ITP. |
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Authors:
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P F Fogarty; M E Rick; W Zeng; A M Risitano; C E Dunbar; J B Bussel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 133 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-08-21 Completed Date: 2003-10-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 461-6 Citation Subset: IM |
Affiliation:
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Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1652, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Analysis of Variance Case-Control Studies Female Genes, T-Cell Receptor beta* Genetic Variation Humans Immunoglobulin Variable Region / genetics* Male Middle Aged RNA / analysis Receptor-CD3 Complex, Antigen, T-Cell / genetics* Reverse Transcriptase Polymerase Chain Reaction Splenectomy Thrombocytopenia / immunology*, surgery Treatment Failure |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Variable Region; 0/Receptor-CD3 Complex, Antigen, T-Cell; 63231-63-0/RNA |
| Comments/Corrections | |
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