Document Detail

T cell receptor VB repertoire diversity in patients with immune thrombocytopenia following splenectomy.
MedLine Citation:
PMID:  12930375     Owner:  NLM     Status:  MEDLINE    
In recent years, a pathophysiological role for T cells in immune thrombocytopenia (ITP) has been established. We applied cDNA size distribution analysis of the T cell receptor (TCR) beta-variable (VB) complementarity-determining region 3 (CDR3) in order to investigate T cell repertoire diversity among immune thrombocytopenia patients who had either responded or not responded to splenectomy, and compared them to normal controls. ITP patients who had had a durable platelet response to splenectomy showed a mean 2.8 +/- 2.1 abnormal CDR3 size patterns per patient, similar to healthy volunteers (2.9 +/- 2.0 abnormal CDR3 size patterns). In contrast, patients unresponsive to splenectomy demonstrated evidence of significantly more clonal T cell expansions than patients who had responded to splenectomy or controls (11.3 +/- 3.3 abnormal CDR3 size patterns per patient; P < 0.001). Of the VB subfamilies analysed, VB3 and VB15 correlated with response or non-response to splenectomy, each demonstrating oligoclonality in non-responding patients (P < 0.05). These findings suggest that removal of the spleen may lead directly or indirectly to reductions in T cell clonal expansions in responders, or that the extent of T cell clonality impacts responsiveness to splenectomy in patients with ITP.
P F Fogarty; M E Rick; W Zeng; A M Risitano; C E Dunbar; J B Bussel
Related Documents :
1780235 - Serum immunoreactive erythropoietin level: a new parameter for monitoring transfusion m...
10584785 - Mr imaging of the brain: findings in asymptomatic patients with thalassemia intermedia ...
18020065 - The effects of acidosis and hypothermia on blood transfusion requirements following fac...
7629585 - Penile scintigraphy for priapism in sickle cell disease.
24776735 - The predictive value of gastrin levels for the diagnosis of gastric enterochromaffin-li...
3197095 - Somatosensory-evoked potential study in headache patients.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  133     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-21     Completed Date:  2003-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1652, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Analysis of Variance
Case-Control Studies
Genes, T-Cell Receptor beta*
Genetic Variation
Immunoglobulin Variable Region / genetics*
Middle Aged
RNA / analysis
Receptor-CD3 Complex, Antigen, T-Cell / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Thrombocytopenia / immunology*,  surgery
Treatment Failure
Reg. No./Substance:
0/Immunoglobulin Variable Region; 0/Receptor-CD3 Complex, Antigen, T-Cell; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Correlation of serum IL-13 and IL-5 levels with clinical response to Glatiramer acetate in patients ...
Next Document:  In vitro co-stimulation with anti-CD28 synergizes with IL-12 in the generation of T cell immune resp...