Document Detail


T cell receptor VB repertoire diversity in patients with immune thrombocytopenia following splenectomy.
MedLine Citation:
PMID:  12930375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, a pathophysiological role for T cells in immune thrombocytopenia (ITP) has been established. We applied cDNA size distribution analysis of the T cell receptor (TCR) beta-variable (VB) complementarity-determining region 3 (CDR3) in order to investigate T cell repertoire diversity among immune thrombocytopenia patients who had either responded or not responded to splenectomy, and compared them to normal controls. ITP patients who had had a durable platelet response to splenectomy showed a mean 2.8 +/- 2.1 abnormal CDR3 size patterns per patient, similar to healthy volunteers (2.9 +/- 2.0 abnormal CDR3 size patterns). In contrast, patients unresponsive to splenectomy demonstrated evidence of significantly more clonal T cell expansions than patients who had responded to splenectomy or controls (11.3 +/- 3.3 abnormal CDR3 size patterns per patient; P < 0.001). Of the VB subfamilies analysed, VB3 and VB15 correlated with response or non-response to splenectomy, each demonstrating oligoclonality in non-responding patients (P < 0.05). These findings suggest that removal of the spleen may lead directly or indirectly to reductions in T cell clonal expansions in responders, or that the extent of T cell clonality impacts responsiveness to splenectomy in patients with ITP.
Authors:
P F Fogarty; M E Rick; W Zeng; A M Risitano; C E Dunbar; J B Bussel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  133     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-21     Completed Date:  2003-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Affiliation:
Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1652, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Case-Control Studies
Female
Genes, T-Cell Receptor beta*
Genetic Variation
Humans
Immunoglobulin Variable Region / genetics*
Male
Middle Aged
RNA / analysis
Receptor-CD3 Complex, Antigen, T-Cell / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Splenectomy
Thrombocytopenia / immunology*,  surgery
Treatment Failure
Chemical
Reg. No./Substance:
0/Immunoglobulin Variable Region; 0/Receptor-CD3 Complex, Antigen, T-Cell; 63231-63-0/RNA
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